ISSN:
1573-5036
Keywords:
culture system
;
ectomycorrhizae
;
extramatrical hyphae
;
Hebeloma crustuliniforme
;
hyphal mass
;
in vitro physiology
;
ponderosa pine
;
Root-Mycocosm
;
synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract A culture system is described to grow mycorrhizal plants which allows experimental measurements to be made on mycorrhizae, and a portion of intact ectomycorrhizal fungi while in symbiosis, but growing apart from the rooting medium and host roots. A portion of the extramatrical hyphae is kept apart from the rooting medium by a restrictive passageway between the root and fungal chambers. The passageway is small enough to restrict coniferous fine roots to the root chamber, but large enough to allow fungal hyphae to grow out of the root chamber onto pre-weighed glass fiber filter paper situated in the fungal chambers. The pre-weighed filter paper allows for gravimetric estimation of the hyphal mass in the fungal chamber. The pieces of the modular Root-Mycocosm can be assembled in various configurations with one or two seedling chambers and one, two or three fungal chambers. Ponderosa pine (Pinus ponderosa Laws.) seedlings were inoculated withHebeloma crustuliniforme (Bull.: St. Amans) Qúel in either commercial-vermiculite inoculum or in plastic growth-pouches and grown in the Root-Mycocosm. Hyphae were allowed to grow into the fungal chambers and after four weeks, amounted to 5.5±0.81 SE and 6.4±1.5 SE mg for pouch and commercial inoculum techniques, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02202096
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