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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of bovine zonae pellucidae from ovaries with collagenase: Antigenic and sperm receptor properties (1979)
    New York, NY : Wiley-Blackwell
    Gamete Research 2 (1979), S. 187-192 
    Details
    ISSN: 0148-7280
    Keywords: zona isolation ; collagenase ; receptor for sperm ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Zonae pellucidae were collected from bovine ovaries by chopping, dispersing the chopped tissue with collagenase, sieving through nylon mesh screens, and pipetting. The zonae were free of corona cell processes when examined under the scanning electron microscope. Solutions of zonae obtained with collagenase exhibited the same antigenic and sperm receptor properties as those obtained without enzyme treatment.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120020210
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of zona antigen in bovine ovary sections by fluorescent antibody (1979)
    New York, NY : Wiley-Blackwell
    Gamete Research 2 (1979), S. 201-206 
    Details
    ISSN: 0148-7280
    Keywords: zona pellucida ; antibody ; ovary ; calf ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Treatment of bovine ovary sections with rabbit antibovine zona serum followed by fluorescein-conjugated goat antirabbit IgG produced a specific fluorescent staining only of the zonae pellucidae. Fluorescence was greater near the inner and outer surface of the zona, suggesting that either the same antigen occurs in higher concentration in these regions or that there is more than one antigen, the most immunogenic being located peripherally. In some atretic follicles fluorescent material appeared to diffuse into the degenerating oocyte and into the intercellular spaces of the cumulus oophorus.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120020303
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    Paper (German National Licenses)
    Fulltext
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