Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
FEMS microbiology letters
21 (1984), S. 0
ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract During aggregation of Dictyostelium discoideum, amoebae move towards collecting centres emitting cyclic AMP. Previous work has indicated that Ca2+ can affect the operation of various parts of the chemotactic mechanism but there are contradictory reports about the role or requirement for Ca2+ during aggregation of intact amoebae. In this study we show that there is a requirement for Ca2+ during this aggregation process. Addition of EGTA (7 mM) and in some cases extensive washing with EGTA is needed to see inhibition of aggregation. Considerable stores of Ca2+ present within cells may explain the failure of some previous attempts to see a Ca2+ requirement during aggregation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1984.tb00179.x
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