ISSN:
1432-203X
Keywords:
Key words:Atropa belladonna—Agrobacterium rhizogenes— Hairy root cultures — Tropane alkaloids
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Abbreviations:ANOVA, analysis of variance; B5 medium, Gamborg B5 medium; BA, N6-benzyladenine; B.S., Balanced Solution; dw, dry weight; EC, electric conductivity; fw, fresh weight; GC/MS, gas chromatography-mass spectrometry; HPLC, high performance liquid chromatography; MS medium, Murashige and Skoog medium; NAA, naphthalene-1-acetic acid; PCR, polymerase chain reaction; SDS, sodium dodecyl sulfate; TMS, trimethylsilyl; WP medium, Woody Plant medium
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. Hairy root cultures of Atropa belladonna were established by transformation with Agrobacterium rhizogenes 15834. Five clones of them were employed to study the production of hyoscyamine, the main constituent of the plant, together with other tropane alkaloids. The growth and alkaloid production of each clone were differently affected by basal liquid culture media tested. The transgenic plants regenerated from each clone of the hairy roots had different phenotypes and diverse alkaloid productivity both in the cultured condition and in hydroponics.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01088281
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