ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The amino acid sequence of a polypeptide was determined by a new method combined with field desorption (FD) mass spectrometry and Edman degradation. The polypeptide, N-terminal BrCN fragment of Streptomyces erythraeus lysozyme with 55 amino acid residues, was cleaved specifically into several peptide fragments by trypsin and chymotrypsin. The molecular weights of subpeptides were determined by FD mass spectrometry. Peptide mixtures were subjected to Edman degradation successively and PTH amino acids released were measured by high performance liquid chromatography. The amino acid sequence was calculated by a computer program from molecular weights of subpeptides and PTH amino acids.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200090204
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