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  • 1
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    A comparative study on the induced breeding and in vitro fertilization performance by various inducing agents in catfish, Heteropneustes fossilis (Bloch) (2021)
    In:  http://aquaticcommons.org/id/eprint/18895 | 12051 | 2015-11-28 14:09:36 | 18895 | Bangladesh Fisheries Research Institute
    Details
    Publication Date: 2021-07-12
    Description: Brood catfish, Heteropneustes fossilis were collected from Tamirabarani river basin of Tamil Nadu, India and kept in cement tanks. Three inducing hormones viz, Ovaprim, Ovatide and WOVA-FH were injected at the rate of 0.5, 1.0, 1.5 and 2.0 ml/kg body weight in order to induce oocyte maturation and ovulation. After 10-13h of injection at a water temperature of 27±-0.5°C, stripping of eggs and in vitro fertilization was done. Ovaprim gave maximum (94.67%) hatching rate followed by Ovatide (90.33%) and WOVA-FH (77.33%).
    Keywords: Fisheries ; induced breeding ; fertilization ; Heteropneustes fossilis
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 1-5
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  • 2
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    Production of triploid African catfish, Clarias gariepinus (Burchell), using chromosome manipulation techniques (2021)
    In:  http://aquaticcommons.org/id/eprint/17658 | 12051 | 2015-09-16 00:07:11 | 17658 | Bangladesh Fisheries Research Institute
    Details
    Publication Date: 2021-06-26
    Description: An experiment was conducted to induce triploidy in African catfish, Clarias gariepinus,using heat shock and cold shock techniques. Cold shock at a temperature of 0± 1°C and5±1°C for a duration of 15, 30, 45 and 60 min and heat shock at a temperature of40±0.5°C and 41 ±OS C for a duration of 1, 2 and 3 min was given to induce triploidy 5min after fertilization. Maximum percentage of triploids (91.4%) were obtained in theheat shock at a temperature of 40±0SC for a duration of 1 min whereas cold shock at0± 1 C for a duration of 60 min yielded 90% of triploids. Chromosome analysis revealedthat diploids have 54 chromosomes and triploids have 81 chromosomes. The erythrocytemeasurements of the minor axis and major axis were 1.17 times larger in treated fishthan in controls. The growth studies showed that the growth rate was not significantlyaffected in triploids.
    Keywords: Aquaculture ; Fisheries ; Triploid ; Chromosome manipulation ; Clarias gariepinus
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 121-130
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  • 3
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    Comparison of effectiveness of heat and cold shocks applied in the induction of gynogenesis in Clarias gariepinus (Burchell) (2021)
    In:  http://aquaticcommons.org/id/eprint/17682 | 12051 | 2015-09-16 00:09:53 | 17682 | Bangladesh Fisheries Research Institute
    Details
    Publication Date: 2021-06-27
    Description: An experiment was conducted to optimize the procedure of gynogenesis in African catfish, Clarias gariepinus by suppressing meiotic and mitotic cell divisions in fertilized eggs. Gynogensis was conducted by fertilizing normal eggs with UV-irradiated sperm followed by either heat or cold shocking Irradiation of spermatozoa was given for a duration of 1 min and the eggs were fertilized in vitro. Cold shock at a temperature of3± 1°C for a duration of 30 and 60 min and heat shock at a temperature of 39± 1°C for a duration of 1 and 2 min was applied to induce diploidy. Higher percentage of hatching (68.66) was observed for meiotic gynogens at a shock temperature of 39± 1°C for a duration of 1 min, 5 min after fertilization (af). Higher percentage of mitotic gynogenetic induction (15.33) was observed at a temperature shock of 39± 1°C for a duration of 1 min, 30 min af.
    Keywords: Aquaculture ; Fisheries ; Gynogenesis ; Sperm irradiation ; Heat shock ; Cold shock
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 131-140
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