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  • 1
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    Globalization and development : a Latin American and Caribbean perspective (2003)
    Palo Alto, Calif : Stanford Social Sciences, Stanford University Press
    Latin American development forum  
    Details
    Keywords: Caribbean Area, Economic conditions. ; Caribbean Area, Foreign economic relations. ; Latin America, Economic conditions. ; Latin America, Foreign economic relations. ; Economic development. ; Globalization.
    Pages: xv, 214 p.
    ISBN: 0-585-47966-6
    URL: http://www.netLibrary.com/urlapi.asp?action=summary&v=1&bookid=100191
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    E-Book (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Changes in composition of conidia of Penicillium notatum during germination (1974)
    Springer
    Archives of microbiology 97 (1974), S. 39-50 
    Details
    ISSN: 1432-072X
    Keywords: Spores ; Conidia ; Germination ; Penicillium ; Chemical Composition ; Germ-Tube ; Mycelium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. The total nitrogen content of spores of Penicillium notatum increased during swelling and reached a maximum before germ-tubes were formed. It subsequently decreased during germ-tube formation until a constant value in hyphae was reached. 2. An increase in the protein content of the spores was found during swelling, followed by a decrease when germ-tubes were formed. After germination, the protein content increased again to a constant level in hyphae. 3. The content of total lipids steadily decreased during swelling of spores. It reached a minimum value in germinating spores, followed by a net accumulation during hyphal growth. Similar changes occurred in free lipids, phospholipids and the acetone-soluble lipids but not in bound lipids. 4. After an initial decrease during swelling, no further change took place in the neutral carbohydrates content of the spores at the time of germ-tube formation. An accumulation of neutral carbohydrates occurred during late hyphal extension. 5. The nucleic acid content increased sharply during swelling, and reached the highest value in swollen spores just prior to the initiation of germ-tube formation. Afterwards, its content steadily decreased during germ-tube formation and hyphae elongation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403043
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  • 3
    Electronic Resource
    Electronic Resource
    Autonomously replicating plasmids carrying theAMA1 region inPenicillium chrysogenum (1996)
    Springer
    Current genetics 29 (1996), S. 482-489 
    Details
    ISSN: 1432-0983
    Keywords: Filamentous fungi ; Origins of replication ; Stability ; Plasmid multimers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plasmid vectors containing theAMA1 sequence transformed with high efficiency and replicated autonomously inPenicillium chrysogenum. The efficiency of transformation ofP. chrysogenum was related to the length of theAMA1 fragment used for constructing the different autonomously replicating plasmids. One of the two palindromic inverted repeats ofAMA1 (the 2.2-kbSalI-HindIII fragment) is sufficient to confer autonomous replication and a high transformation efficiency. Deletion of the 0.6-kb central fragment located between the inverted repeats did not affect either the ability of the plasmids to replicate autonomously or the efficiency of transformation, but did alter the mitotic stability and the plasmid copy number. Deletion of any fragment of the 2.2-kb repeat caused the loss of the ability to replicate autonomously and reduced the transformation efficiency. Most of the transformants retained the original plasmid configuration, as multimers and without reorganization, after several rounds of autonomous replication. TheAMA1 region works as an origin of replication inP. chrysogenum andA. nidulans but not apparently inAcremonium chrysogenum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221518
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization and nitrogen-source regulation at the transcriptional level of the gdhA gene of Aspergillus awamori encoding an NADP-dependent glutamate dehydrogenase (1998)
    Springer
    Current genetics 34 (1998), S. 50-59 
    Details
    ISSN: 1432-0983
    Keywords: Key words Glutamate dehydrogenase ; Nitrogen regulation ; Aspergillus awamori ; Chromosome resolution ; Promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 28.7-kb DNA region containing the gdhA gene of Aspergillus awamori was cloned from a genomic DNA library. A fragment of 2570 nucleotides was sequenced that contained ORF1, of 1380 bp, encoding a protein of 460 amino acids (Mr 49.4 kDa). The encoded protein showed high similarity to the NADP-dependent glutamate dehydrogenases of different organisms. The cloned gene was functional since it complemented two different Aspergillus nidulans gdhA mutants, restoring high levels of NADP-dependent glutamate dehydrogenase to the transformants. The A. awamori gdhA gene was located by pulsed-field gel electrophoresis in a 5.5-Mb band (corresponding to a doublet of chromosomes II and III), and was transcribed as a monocistronic transcript of 1.7 kb. Transcript levels of the gdhA gene were very high during the rapid growth phase and decreased drastically after 48 h of cultivation. Very high expression levels of the gdhA gene were observed in media with ammonium or asparagine as the nitrogen source, whereas glutamic acid repressed transcription of the gdhA gene. These results indicate that expression of the gdhA gene is subject to a strong nitrogen regulation at the transcriptional level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050365
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  • 5
    Electronic Resource
    Electronic Resource
    Construction of new cloning vectors for Brevibacterium lactofermentum (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 137 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Two plasmid cloning vectors (pULMJ55 and pULMJ95) were constructed for Brevibacterium lactofermentum using the origin of replication of the endogenous plasmid pBL1. Plasmid pULMJ55 is a replacement vector with transcriptional terminators from the B. lactofermentum trp operon flanking the BglII cloning sites. Religation of the BglII digested vector without insert creates a 376 bp perfect palindrome that is not tolerated in B. lactofermentum, giving positive selection for recombinant plasmids with inserts. Plasmid pULMJ95 contains the promoter-less α-amylase gene from Streptomyces griseus downstream of the trp terminator and is particularly suitable for the detection of promoters which are activated late during the growth phase. α-Amylase is secreted and its activity can be detected using simple plate tests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08083.x
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  • 6
    Electronic Resource
    Electronic Resource
    Thiostrepton induced proteins in Streptomyces, Amycolatopsis and Nocardia species (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 118 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Thiostrepton-induced proteins have been observed in several actinomycetes, including Streptomyces clavuligerus (15 kDa protein), S. griseus (21 kDa), Nocardia lactamdurans (17 kDa), Amycolatopsis sp 239 (14 and 16 kDa), S. fradie (18 and 20 kDa) and S. coelicolor (17, 19, 30 and 56 kDa). The thiostrepton-induced proteins of S. coelicolor seem to be identical to those reported in S. lividans. The induction of the 18 and 20 kDa proteins of S. fradiae (up to 20% of the total protein in the cells) was dependent upon the concentration of thiostrepton (1 to 50 μg per ml) added to the cultures. The induction of proteins by thiostrepton in N. lactamdurans (and probably in the other actinomycetes as well) is not mediated by the tsr gene of pIJ702, since spontaneous thiostrepton-resistant mutants of N. lactamdurans which do not carry the tsr gene were used for the induction studies. Induction of proteins by thiostrepton may be a protective mechanism to counteract the strong effect of this antibiotic which is known to be produced by several soil microorganisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06811.x
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  • 7
    Electronic Resource
    Electronic Resource
    Use of a cloned gene involved in candicidin production to discover new polyene producer Streptomyces strains (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 70 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A p-aminobenzoic synthase gene (pabS) from Streptomyces griseus IMRU 3570 involved in candicidin production was used as probe to find new aromatic polyene producing Streptomyces strains. The pab gene hybridizes with 6 out 16 Streptomyces strains, and those strains which hybridize turned out to be polyene producers. Such strains were never before described as polyene producers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb03768.x
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  • 8
    Electronic Resource
    Electronic Resource
    A bifunctional Streptomyces-E. coli promoter-probe vetor (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 68 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A bifunctional Streptomyces-E. coli promoter probe vector, pULJA30, has been developed to isolate and characterize nucleotide sequences involved in transcription initiation and regulation. The vector is derived from plasmid pIJ486 [1], carries the pIJ101 replicon and utilizes the promoterless amoniglycoside phosphotransferase (neo) as indicator gene. Important features of the new vector include: wide Streptomyces host range and as high a plasmid copy number as the parental pIJ486, an upstream transcriptional terminator (toop) and a polylinker sequence with unique sites for BamHI and BglII for flexible cloning, fragment re-isolation and direct sequencing of promoter-active inserts. pULJA30 also has an E. coli replicon (from pBR322) and the possibility of selection in Streptomycers and E. coli by using the tsr, neo and bla genes, which makes it very convenient to test the comparative functionality of Streptomyces promoters in E. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb04123.x
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  • 9
    Electronic Resource
    Electronic Resource
    Targeted integration of foreign genes into repetitive sequences of the Brevibacterium lactofermentum chromosome (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 142 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Directed integration of heterologous DNA into the insertion sequence IS13869 of Brevibacterium lactofermentum has been achieved by using integrative vectors that contain a 600 bp fragment from IS13869 and a kanamycin resistance cartridge with unique PacI, SwaI and PmeI sites. The incorporation of these restriction sites for rare-cutting enzymes, which recognize octanucleotide sequences, allowed rapid mapping of the location of plasmid insertion into the chromosome. Mapping by pulsed-field gel electrophoresis and hybridization with an IS13869 probe showed four types of transformants. In three of these types, integration of the vector occurred at each of three different sites in the chromosome. These vectors have the advantage of increasing the number of integration events due to the presence of multiple targets in the genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08440.x
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis of the codon usage of the cephamycin C producer Nocardia lactamdurans (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 110 (1993), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The G + C content in a sequenced region of 27 kb of the Nocardia lactamdurans genome is 70.4 and 70.6% in the 14 characterized ORFs, showing an extreme average G + C content (94.9%) in the third codon position. The codon usage parameters of the N. lactamdurans genes studied are closely related and depart weakly from the values of other species of the genus Nocardia. The homologies and differences in the codon usage between N. lactamdurans and Streptomyces sp. or other high-G + C Gram-positive genera are analysed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06300.x
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