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  • 1
    Electronic Resource
    Electronic Resource
    Growth and Cardenolide Production by Digitalis lanata in Different Fermentor Types (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 434 (1984), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1984.tb29877.x
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  • 2
    Electronic Resource
    Electronic Resource
    Nutritional regulation of proteinase production in the fungus, Tritirachium album (2000)
    Springer
    Journal of industrial microbiology and biotechnology 24 (2000), S. 369-373 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: proteinase; regulation; fungus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The fungus, Tritirachium album, produces a number of proteinases under proper conditions. We have studied the nutritional regulation mechanisms for proteinase production in the mold, i.e. the effects of carbon and nitrogen sources, and the influence of starvation. Proteinase production was induced when the nitrogen source was an exogenous protein or peptide, such as peptones or yeast extract. The production rate was affected by the amount of available inducing substrate. Inorganic nitrogen compounds, i.e., ammonium or nitrate salts, had a repressing effect on the production. Production was not induced if a detectable concentration of glucose or sucrose was present in the medium. Starvation did not trigger proteinase production. Journal of Industrial Microbiology & Biotechnology (2000) 24, 369–373.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.7000012
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  • 3
    Electronic Resource
    Electronic Resource
    Escherichia coli plasmid production in fermenter (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 386-393 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Escherichia coli harboring a recombinant plasmid was grown in a fermenter to study the effects of selected process parameters on the growth of the microbe and on plasmid amplification with chloramphenicol treatment. Eighteen fermentations were carried out according to a statistical experimental design in which the fermentation temperature, pH, and turbidity of culture at the onset of plasmid amplification were the selected independent process variables. Static regression models describing the process were derived from the experimental results. It turned out that recombinant plasmid copy numbers could be influenced by controlling fermentation temperature and pH. The maximal copy number during bacterial growth phase and the optimal plasmid production were found to require fermentation conditions different from those needed for optimal bacterial growth and cell division. The conditions also differed significantly from those routinely used in research laboratories for plasmid preparation. The chloramphenicol treatment increased the plasmid copy number compared with chromosome numbers up to fivefold. Some of the data suggest that under certain conditions the number of chromosome molecules in E. coli cells may rise during the plasmid amplification stage. Statistical experimental design, a nucleic acid sandwich hybridization technique for plasmid quantification, and regression models proved to be useful in this study.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260330403
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  • 4
    Electronic Resource
    Electronic Resource
    Studies on growth and cardenolide production of Digitalis lanata tissue cultures (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 558-562 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Digitalis lanata cell cultures grown as small undifferentiated aggregates in suspension culture can be redifferentiated into green embryos that produce cardenolides. The possibility of using a statistical (Box-Wilson) experimental design to study the effects of four different variables on growth, differentiation, and cardenolide production of D. lanata tissue cultures are investigated. The results of the analyses were processed by linear regression analysis. Mathematical models explaining the effects of the variables were developed. The concentration of maltose and the NO3-—NH4+ ratio were found to be significant variables for both growth and cardenolide production. The size of the inoculum was also important.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260330507
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