ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Sirolimus-loaded poly (lactic acid-co-glycol acid) (PLGA) films were prepared by acasting method. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay wasperformed to determine the optimal concentration of sirolimus for vascular smooth muscle cell(VSMC) inhibition effect. Based on the results of MTT, three samples of different weight percentloaded-sirolimus (2wt%, 6wt%, 15wt%) PLGA films were selected. The release of lactatedehydrogenase (LDH) was used to evaluate the immediate toxicity of the sirolimus-loaded PLGAfilms and it was shown that the three different concentrations of sirolimus-loaded films have lowtoxicity. Alamar blue results indicate that the sirolimus-loaded films have better antiproliferationeffect than 316 stainless steel (SS). The higher the sirolimus concentration in the film is, the better isthe antiproliferation effect. These films may be used for stent coating to inhibit in-stent restenosisinduced by VSMC proliferation
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.837.pdf
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