ISSN:
1476-5535
Keywords:
Citrobacter sp.
;
Immobilized cells
;
Low pH
;
Lanthanum removal
;
Lanthanum phosphate solubility
;
Lanthanum phosphate desolubilization
;
Bioreactor kinetics
;
Phosphatase activity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary A biotechnological process for the uptake of metals from solution was evaluated at low pH. Metal uptake is mediated by the enzymatic liberation of phosphate by immobilized cells of aCitrobacter, insoluble metal phosphate is retained at the cell surface. Lanthanum uptake was abolished at pH 4, but the activity of the mediating phosphatase was reduced by only 50% at this pH. At pH 5 phosphate release was largely unaffected as compared to that at pH 7, but La removal was reduced. Growth of the strain under conditions which gave a four-fold increase in phosphatase activity gave only a two-fold increase in metal removal at pH 5 by immobilized cells. The precipitated species was identified as LaPO4; the poor removal seen at low pH was attributed to inefficient desolubilization of lanthanum phosphate. In addition, some contributory biochemical factors were identified. The apparentK m (K m app) of the whole-cell enzyme forp-nitrophenyl phosphate was little-affeted by the pH, but theK m app for glycerol 2-phosphate was increased at pH 5. This influences the activity of the bioreactor at high flow rates and is reflected in the gradient seen on plots of the in flow rate-activity relatioship, in accordance with a Michaelis-Menten description of the bioreactor. It is concluded that althougt reduced metal uptake at low pH is mainly attributable to chemical effects, these can be exacerbated according to the choice of substrate supporting the desolubilization reaction:p-nitrophenyl phosphate is the better substrate for efficient metal desolubilization at high flow rates at low pH values.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01569939
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