Publication Date:
1984-03-30
Description:
The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization. No functional differences between native and the recombinant interleukin-2 molecules have been detected.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rosenberg, S A -- Grimm, E A -- McGrogan, M -- Doyle, M -- Kawasaki, E -- Koths, K -- Mark, D F -- New York, N.Y. -- Science. 1984 Mar 30;223(4643):1412-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6367046" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
Cell Line
;
DNA, Recombinant/metabolism
;
Escherichia coli/*metabolism
;
Humans
;
Interleukin-2/biosynthesis/*genetics/physiology
;
Killer Cells, Natural/physiology
;
Lymphocytes/physiology
;
Mice
;
Mice, Inbred C57BL
;
*Recombination, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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