Publication Date:
2009-09-26
Description:
Metabolic plasticity, which largely relies on the creation of new genes, is an essential feature of plant adaptation and speciation and has led to the evolution of large gene families. A typical example is provided by the diversification of the cytochrome P450 enzymes in plants. We describe here a retroposition, neofunctionalization, and duplication sequence that, via selective and local amino acid replacement, led to the evolution of a novel phenolic pathway in Brassicaceae. This pathway involves a cascade of six successive hydroxylations by two partially redundant cytochromes P450, leading to the formation of N1,N5-di(hydroxyferuloyl)-N10-sinapoylspermidine, a major pollen constituent and so-far-overlooked player in phenylpropanoid metabolism. This example shows how positive Darwinian selection can favor structured clusters of nonsynonymous substitutions that are needed for the transition of enzymes to new functions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Matsuno, Michiyo -- Compagnon, Vincent -- Schoch, Guillaume A -- Schmitt, Martine -- Debayle, Delphine -- Bassard, Jean-Etienne -- Pollet, Brigitte -- Hehn, Alain -- Heintz, Dimitri -- Ullmann, Pascaline -- Lapierre, Catherine -- Bernier, Francois -- Ehlting, Jurgen -- Werck-Reichhart, Daniele -- New York, N.Y. -- Science. 2009 Sep 25;325(5948):1688-92. doi: 10.1126/science.1174095.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut de Biologie Moleculaire des Plantes, CNRS-UPR2357, Universite de Strasbourg, 28 Rue Goethe, 67083 Strasbourg Cedex, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19779199" target="_blank"〉PubMed〈/a〉
Keywords:
Arabidopsis/genetics/metabolism
;
Base Sequence
;
Brassica napus/genetics/growth & development/metabolism
;
Brassicaceae/genetics/growth & development/*metabolism
;
Cytochrome P-450 Enzyme System/chemistry/genetics/*metabolism
;
*Evolution, Molecular
;
Gene Duplication
;
Hydroxylation
;
Metabolic Networks and Pathways
;
Methylation
;
Models, Molecular
;
Molecular Sequence Data
;
Plant Proteins/chemistry/genetics/metabolism
;
Pollen/*growth & development/metabolism
;
RNA Interference
;
Retroelements
;
Selection, Genetic
;
Spermidine/*analogs & derivatives/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink