Publication Date:
2010-06-26
Description:
The lower intestine of adult mammals is densely colonized with nonpathogenic (commensal) microbes. Gut bacteria induce protective immune responses, which ensure host-microbial mutualism. The continuous presence of commensal intestinal bacteria has made it difficult to study mucosal immune dynamics. Here, we report a reversible germ-free colonization system in mice that is independent of diet or antibiotic manipulation. A slow (more than 14 days) onset of a long-lived (half-life over 16 weeks), highly specific anticommensal immunoglobulin A (IgA) response in germ-free mice was observed. Ongoing commensal exposure in colonized mice rapidly abrogated this response. Sequential doses lacked a classical prime-boost effect seen in systemic vaccination, but specific IgA induction occurred as a stepwise response to current bacterial exposure, such that the antibody repertoire matched the existing commensal content.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923373/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923373/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hapfelmeier, Siegfried -- Lawson, Melissa A E -- Slack, Emma -- Kirundi, Jorum K -- Stoel, Maaike -- Heikenwalder, Mathias -- Cahenzli, Julia -- Velykoredko, Yuliya -- Balmer, Maria L -- Endt, Kathrin -- Geuking, Markus B -- Curtiss, Roy 3rd -- McCoy, Kathy D -- Macpherson, Andrew J -- R01 AI060557/AI/NIAID NIH HHS/ -- Canadian Institutes of Health Research/Canada -- New York, N.Y. -- Science. 2010 Jun 25;328(5986):1705-9. doi: 10.1126/science.1188454.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉DKF (Maurice Muller Laboratories), MEM, Universitatsklinik fur Viszerale Chirurgie und Medizin (UVCM), University of Bern, 3013 Bern, Switzerland. hapfelmeier@gmail.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20576892" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Bacterial/biosynthesis/*immunology
;
Antibody Specificity
;
Colony Count, Microbial
;
Dose-Response Relationship, Immunologic
;
Escherichia coli/*growth & development/*immunology
;
Germ-Free Life
;
Half-Life
;
Immunoglobulin A/biosynthesis/*immunology
;
Immunologic Memory
;
Intestinal Mucosa/*immunology/*microbiology
;
Intestines/immunology/microbiology
;
Mice
;
Mice, Inbred C57BL
;
Mucous Membrane/immunology
;
Plasma Cells/immunology
;
Time Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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