ISSN:
1573-5060
Keywords:
Dactylis glomerata L
;
orchardgrass
;
tissue culture
;
plant regeneration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Shoot development through morphological transformation in spikelets occurred after segments of young unemerged orchardgrass (Dactylis glomerata L.) inflorescences were cultured on Linsmaier and Skoog's RM medium supplemented with 0, 4.52×10-4, 4.52×10-3 and 2.26×10-2 mM 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthaleneacetic acid (NAA). Effects on shoot formation were better with 2,4-D than NAA in all concentrations tested. The callus initiated from the primary culture on high 2,4-D medium was reproducible, but no evidence of shoot proliferation was noted. The shoots developed into healthy plantlets after being reared on RM medium not supplemented with hormones.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00028302
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