ISSN:
1432-1130
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A new method based on the Q parameter, that permits the determination of the Ccompound A /Ccompound B ratio without preparing calibration graphs of the two compounds, is proposed. This method has been applied to signals obtained by synchronous luminiscence. Simultaneous determination of α-tocopherol and α-tocopheryl acetate in beverages using synchronous fluorescence has been carried out. To isolate the compounds from samples, liquid extraction with n-hexane as the organic phase was employed. The presence of interferences was tested using the apparent content curves (ACC) method and the Cα-tocopherol/¶Cα-tocopheryl acetate ratio was calculated using the Q parameter. The reproducibility and detection limit for the determination of α-tocopherol and α-tocopheryl acetate were 6.6% and 0.016 mg/L and 1.8% and 0.017 mg/L, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002160000354
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