ISSN:
1617-4623
Keywords:
Deletion
;
DNA sequencing
;
Subtilisin
;
Serine protease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A multipurpose broad host range plasmid, pIJ3200, was constructed by inserting the polylinker-containing 445 by PvuII fragment of Bluescript M13 into the EcoRI site of the cosmid pLAFR1. Using this vector a protease gene of Xanthomonas campestris pathovar campestris, previously cloned in the recombinant plasmid pIJ3070, was located by deletion to a 2.2 kb DNA region. Sequencing of the protease gene revealed an open reading frame encoding a 580 amino acid polypeptide with molecular weight of 57000. The deduced amino acid sequence showed strong homology with the subtilisin family of serine proteases. This, together with its sensitivity to inhibition by phenylmethylsulphonyl fluoride, suggests that the enzyme belongs to this family of proteases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00391750
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