ISSN:
1612-1112
Keywords:
Capillary electrophoresis
;
Serum analysis
;
Total iron binding capacity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A capillary electrophoresis (CE) technique for determining total iron binding capacity (TIBC) of serum has been developed. The optimum serum pretreatment involves the following major steps: at first, saturate serum transferrin with Fe+3; then, dissociate them completely after removing excess unbound Fe. Finally, complex the released iron with phenanthroline, a chromophore, to make suitable for the CE analysis. Ammonium acetate (pH=5.0) was used as CE background electrolyte solution. In this system, a good linear correlation coefficient was maintained over the range 0.5≈10 μM (r=0.9979,n=12). Seven adult serum samples were studied and the TIBC parameters measured. In the present system, 10≈30 μL serum is sufficient for determination. The study shows that the CE technique described is a powerful method for rapid, efficient, sensitive and reliable analysis and hence particularly suitable for clinical application.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02467190
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