ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Biodegradable nanofiber has become a popular candidate as tissue engineering scaffolds due to itsbiomimic structure as natural extracellular matrix (ECM). Certain tissue regeneration may requireprolonged in vitro culture time for cellular reorganization and tissue remodeling. Therefore, longterm understanding of cellular effects on scaffold degradation is needed. Although there are somedegradation studies on nanofiber, degradation study of nanofibers with cell culture is rare. In ourstudy, polyglycolide (PGA), poly(DL-lactide-co-glycolide) (PLGA), poly(L-lactide-co-ε-caprolactone) [P(LLA-CL)] were electrospun into nanofiber scaffolds. The scaffolds were culturedwith porcine smooth muscle cells (PSMC) for up to 3 months to evaluate their degradation behaviorand cellular response. The results showed that their degradation rates were in the order ofPGA〉〉PLGA〉P(LLA-CL). PGA nanofiber degraded in 3 weeks and only supported cell growth inthe first few days. Cell culture accelerated the surface erosion of PLGA and P(LLA-CL) nanofiberwhile the bulk degradation remained unaffected. Furthermore, the cell culture did not significantlyreduce the mechanical strength of PLGA and P(LLA-CL) during degradation
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/41/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.47-50.1327.pdf
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