ISSN:
0263-6484
Keywords:
oxidized low-density lipoprotein
;
nitric oxide
;
mesangial cells
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Recently, the close relation between oxidized low density lipoprotein (Ox-LDL) and the progression of glomerular injury has been demonstrated. The nitric oxide (NO) pathway in glomerular mesangial cells may be a potential target for the adverse effects of Ox-LDL in the development of glomerular injury. In this study, we treated cultured rat mesangial cells (RMC) with Fe2+-oxidized LDL and then stimulated the cells with lipopolysacharride (LPS, 10 μg ml-1). The LPS-induced NO production, assessed by NO2- concentrations in cultured supernatants, decreased from 7·83 nmol per 106 cells in control to 4·00 nmol per 106 cells and 1·67 nmol per 106 cells in RMC preincubated with Ox-LDL at 20 μg ml-1 and 40 μg ml-1, respectively (P〈0·01). Native LDL had no significant effects on LPS-induced NO production. Using the reverse transcription-polymerase chain reaction (RT-PCR) technique, we could not detect significant alteration of inducible NO synthase (iNOS) mRNA levels in RMC preincubated with Ox-LDL. Our results suggest that Ox-LDL decreases induced NO production in RMC, which may contribute to the adverse effects of Ox-LDL in progressive glomerular injury. The mechanisms of this decrease may not involve changes of iNOS genic transcription. © 1998 John Wiley & Sons, Ltd.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
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