ISSN:
1432-072X
Keywords:
Lactobacillus plantarum
;
Superoxide dismutase
;
Superoxide anion
;
Pulse radiolysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cell-free extracts of Lactobacillus plantarum contain non-proteinaceous compounds which mimic superoxide dismutase activity. Using the test system in which O 2 − is generated by xanthine oxidase, superoxide dismutase activity is found in cell-free extracts, where proteins are removed by precipitation. This activity is strongly decreased after dialysis of cell-free extracts. Superoxide dismutase activity was also investigated by means of pulse radiolysis. Cell-free extracts of Escherichia coli were also investigated as a comparison, which were known to contain superoxide dismutase. With cell-free extracts of both L. plantarum and E. coli the decay of O 2 − was markedly increased. However, the type of reaction of the O 2 − decay was of first order in the presence of E. coli extracts due to superoxide dismutase(s), and of second order in the presence of L. plantarum extracts, indicating that O 2 − elimination is not an enzymic reaction. Mn2+ phosphate(s) might be responsible for the observed elimination of O 2 − . The production of O 2 − is not detectable during NADH-, lactate- or pyruvate oxidase reactions in L. plantarum extracts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446879
Permalink