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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 86 (1973), S. 65-75 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Le transfert graduel d'eau douce en eau de mer de l'Anguille argentée n'entraîne pas de variations très significatives de Na+ et K+ plasmatiques étudiés aux 10, 20, 30 et 50e jours; la calcémie s'abaisse régulièrement alors que la chlorémie s'accroît. L'hypophysectomie, réalisée 24 h avant le transfert, ne modifie pas les réponses trouvées chez l'Anguille intacte en fonction de la durée du séjour en eau de mer. La prolactine ovine injectée chez l'Anguille intacte (10 j) ou hypophysectomisée (20, 30 et 50 j) induit une augmentation très significative des électrolytes étudiés. Ces résultats sont comparés à ceux concernant l'activité de la Na+-K+-ATPase branchiale, intestinale et rénale. Si une corrélation entre cette activité enzymatique et la différence entre Na+ externe et Na+ plasmatique est évidente lors de l'augmentation de la salinité, elle n'est plus apparente après hypophysectomie et/ou traitement avec la prolactine en eau de mer.
    Notes: Summary Male silver eels were gradually transferred from fresh water to sea water. Plasma Na+ and K+ levels are not significantly affected after 10, 20, 30 and 50 days, plasma Ca++ slowly decreases and plasma Cl− increases. Similar electrolyte changes occur in eels hypophysectomized the day before their transfer. Ovine prolactin injected immediately after pituitary ablation induces a marked increase of plasma electrolytes in intact (10 daily injections) or hypophysectomized eels injected for 20, 30 and 50 days. These data were compared with those previously obtained on Na+-K+-ATPase activity in gill filaments, intestinal mucosa and kidney. If there is a positive correlation between gill Na+-K+-ATPase activity and the difference between plasma and environmental sodium concentration when salinity is increased, no such correlation is apparent after hypophysectomy and/or prolactin treatment in sea water. The physiological meaning of prolactin secretion in sea water is discussed.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 73 (1971), S. 34-43 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les variations de la teneur en acide sialique (acide N-acétyl-neuraminique) de la peau d'Anguilla anguitta L. sont étudiées chez des animaux, intacts ou hypophysectomisés, traités avec de la prolactine ovine ou du sérum physiologique, en eau douce. La concentration en acide est évaluée après coloration à l'acide thiobarbiturique et mesure au spectrophotomètre. Chez l'Anguille intacte, l'injection quotidienne de prolactine pendant 11 à 13 jours ne modifie pas le taux d'acide sialique, qui reste identique à celui d'animaux intacts recevant du solvant. L'hypophysectomie entraîne une chute marquée de la concentration en acide N-acétyl-neuraminique. Cette réduction, très significative après 20 jours, s'accentue après 50 jours (40 injections de solvant), en dépit d'une chute passagèrement moindre après 30 jours. L'injection quotidienne de prolactine ovine, débutant immédiatement après l'hypophysectomie, s'oppose à cette chute et permet le maintien d'une teneur en acide légèrement inférieure ou égale à celle des témoins, soit significativement supérieure à celle des hypophysectomisés recevant du solvant. Il existe un parallélisme intéressant entre l'évolution de la teneur en acide sialique de la peau et celle de la natrémie après hypophysectomie et apport de prolactine, étudiées chez les mêmes animaux. Les données publiées concernant l'effet de la prolactine et de l'ablation de l'hypophyse sur la taille et le nombre des cellules à mucus de la peau sont résumées et discutées; ces résultats suggèrent fortement l'idée que le taux de l'acide sialique, constituant du mucus, peut refléter valablement la quantité de mucus cutané. Ils indiquent que le mucus est susceptible de jouer un rôle dans l'osmorégulation de l'Anguille et se trouve soumis à un contrôle prolactinique.
    Notes: Summary Sialic acid (N-acetyl-neuraminic acid) levels were determined after staining with thiobarbituric acid and spectrophotometric measurement, in the skin of intact and hypophysectomized eels, treated with ovine prolactin or solvent (6 per 1000 sodium chloride) in fresh water. No modification of sialic acid content was detected in the skin of intact eels treated with ovine prolactin, the values being similar to those of control eels injected with solvent only. The sialic acid concentration is significantly reduced after hypophysectomy, this effect being more marked after 50 days than after 20, although it is slightly less significant after 30 days; these animals received 15, 25, and 40 solvent injections. Prolactin injections, starting immediately after hypophysectomy, prevent this decrease and maintain a normal or subnormal sialic acid content, higher than that of hypophysectomized animals injected with solvent. There is an interesting parallelism between the variations of sialic acid concentrations of the skin and those of blood sodium levels after hypophysectomy and prolactin treatment, studied in the same animals. Published data on the effect of prolactin and pituitary removal on the size and number of the skin mucous cells are summarized and discussed. Results obtained strongly suggest that sialic acid level, the presence of which was detected in the mucus with histochemical technique, may constitute a valid indication of the amount of cutaneous mucus. It also suggests that mucus may play a role in osmoregulation in the eel and is under prolactin control.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 73 (1971), S. 44-52 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé La teneur en acide N-acétyl-neuraminique du mucus de la peau d'Anguille est mesurée après coloration avec l'acide periodique-acide thiobarbiturique au spectrophotomètre. En eau douce, à 21° C, elle présente une chute très significative de 34,1% 2 mois après hypophysectomie par rapport à celle d'animaux intacts. L'autotransplantation de l'hypophyse dans la musculature dorsale atténue cette chute (19,7%), effet similaire à celui de l'injection de prolactine ovine. Ces réponses de la teneur en acide sialique, utilisées comme indice des variations quantitatives du mucus de la peau, suggèrent que greffe et prolactine ovine stimulent la production de mucus. Or, chez les Téléostéens, divers travaux montrent que l'hypophyse transplantée sécrète une prolactine, probablement responsable de cette action. Ces données sur l'acide sialique sont comparées à celles obtenues sur les électrolytes sériques et la structure rénale après hypophysectomie, autogreffe hypophysaire et injection de prolactine. La similitude des résultats dans ces diverses conditions expérimentales suggère les hypothèses suivantes: la teneur en acide N-acétylneuraminique de la peau serait contrôlée, au moins en partie, par la prolactine; par sa sécrétion de mucus, la peau pourrait être partiellement responsable du maintien de l'équilibre osmotique modifié après hypophysectomie. Enfin, le déficit global observé ne plaide pas en faveur de l'existence d'un facteur hypothalamique inhibiteur de la sécrétion de prolaotine (PIF) chez l'Anguille, malgré l'image histologique d'hyperactivité des éléments prolactiniques dans l'hypophyse déconnectée.
    Notes: Summary The concentration of N-acetyl-neuraminic acid in the skin mucus of the eel was determined by spectrophotometric measurement after staining with periodic acid-thiobarbituric acid. Two months after hypophysectomy, in fresh water at 21° C, a 34.1% decrease, compared with intact animals kept under identical conditions is detected in the skin. When the pituitary gland is autotransplanted in the dorsal muscle, this decrease is limited to 19.7%, an effect which is similar to that of an ovine prolactin treatment. These responses of the sialic acid content, which reflect the quantitative variations of the skin mucus, suggest that both grafts and ovine prolactin stimulate mucus production. In teleost fishes, some results indicate that transplanted pituitary gland is secreting a prolactin-like hormone most probably involved in this effect. These data on the skin sialic acid content are compared with those obtained on blood electrolytes and renal histology after hypophysectomy, pituitary graft and prolactin injection. The similarities of results collected under these various experimental conditions suggest the following hypothesis: the content of cutaneous N-acetyl-neuraminic acid would be, at least in part, under the control of a prolactin hormone. By its mucus production, the skin might be partly responsible for the maintenance of osmotic balance disturbed after hypophysectomy. Finally, the total amount of prolactin secreted by the graft seems to be lower than that released by the in situ gland; this fact is not in favour of the presence of a prolactin-inhibiting factor (PIF) in the hypothalamus of eels, although prolactin cells appear stimulated in the ectopic pituitary gland.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 79 (1972), S. 411-422 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Le transfert graduel de l'Anguille d'eau douce en eau de mer provoque une augmentation de la teneur en acide N-acétyl-neuraminique (ANAN) de la peau, temporaire chez le mâle argenté, prolongée chez le mâle en cours d'argenture. Les cellules à prolactine de l'adénohypophyse, très actives en eau douce, subissent une lente involution, mais après 30 jours en eau de mer, leur aspect n'indique pas encore un hypofonctionnement très marqué. L'évolution de la mucifieation cutanée et celle de l'aspect des cellules à prolactine ne présentent donc pas de corrélation étroite au cours de l'adaptation à l'eau de mer, surtout après 160 jours. Chez les Anguilles maintenues en eau de mer pendant 20 jours, le retour en eau douce affecte légèrement la teneur en ANAN; elle demeurs très supérieure à celle des témoins restés en eau douce. Les cellules à prolaetine sont stimulées, mais même 10 jours après le transfert, l'image obtenue est encore loin de celle initialement observée en eau douce. Ces changements de salinité modifient les electrolytes plasmatiques. Les corrélations entre ces divers résultats sont envisagées.
    Notes: Summary Progressive transfer of European eels from fresh water to sea water induces an increase in the skin content of N-acetyl-neuraminic acid (ANAN). This increase is of short duration in silver male eels but lasts for at least 160 days in animals studied during the silvering process. In the pituitary gland the cells secreting a prolactin-like hormone are very active in fresh water but slowly regress after transfer to sea water. Even after 30 days the regression is only slight but after 160 days they are markedly hypoactive. The development of skin mucus production and the activity of the prolactin cells are not correlated during adaptation to sea water particularly after five months. The transfer back to fresh water of eels which have been kept in sea water for 20 days, produces slight changes in the skin content of ANAN which, after 2, 5 or 10 days, tends to be lower or similar to that of animals remaining in sea water. However, it remains much higher than in controls maintained in fresh water. This transfer stimulates the prolactin cells but even after 10 days their histological picture is different from that commonly observed in fresh water. Plasma electrolytes are affected by the salinity changes and some correlations among histological and biochemical data are considered and discussed in the light of results obtained by other authors on different teleosts.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 95 (1969), S. 361-376 
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les divers segments du rein de l'Anguille hypophysectomisée depuis 6, 11 ou 16 jours conservent les caractères histologiques observés chez l'animal intact. La mesure des hauteurs épithéliales donne des valeurs comparables à celles des animaux intacts, mais les noyaux présentent une surface significativement inférieure après hypophysectomie; de plus, quelques segments proximaux présentent de petites vacuoles qui pourraient correspondre au début de l'involution de ces tubules. Le traitement avec la prolactine ovine induisait d'importantes modifications cytologiques, suggérant une action stimulante sur le rein de l'animal intact; elles se retrouvent en partie après hypophysectomie: la hauteur cellulaire et les diamètres tubulaires des divers segments sont accrus sauf pour le segment collecteur qui réagit peu intensément. La réponse nucléaire est également très réduite, aucune augmentation n'est bien décelable dans les noyaux des divers segments ni même dans le segment collecteur initial, bien que certains nucléoles soient plus nets, dans un nucléoplasme clair. Quelques mitoses se forment dans les tubules et, en outre, de nombreux bourgeons apparaissent, au contact du collecteur et se différencient en tubules, comme chez les Anguilles intactes. Quelques observations concernent le tissu lymphoïde et les cellules juxtaglomérulaires. La prolactine présente donc encore une action en l'absence de l'hypophyse, mais elle est moins intense que celle observée chez l'Anguille intacte. Malgré la conservation d'une euryhalinité excellente, d'autre(s) facteur(s) hypophysaire(s) serai(en)t nécessaire(s) pour que la prolactine manifeste un effet complet. Les différentes modifications sont envisagées en corrélation avec les données concernant les changements d'osmolarité plasmatique ou urinaire et de flux de sodium, obtenues après hypophysectomie chez divers Téléostéens traités ou non avec de la prolactine en eau douce.
    Notes: Summary The histological structure of the kidney in eels hypophysectomized for 6, 11 or 16 days is quite similar to that described previously in intact animals. The values of the epithelial cell height, in the various segments are comparable in intact and hypophysectomized eels, but the nuclear area is significantly reduced in the latter. Simultaneously, some vacuolization of a few tubules occurs in the proximal segment, a process which might suggest the beginning of some degeneration. The treatment with ovine prolactin, which induces evident changes in intact eels, suggesting a stimulating effect, still acts on the kidney after hypophysectomy: cell height and tubule diameter are increased as in normal fish, in the proximal 1 and 2 and the distal segments, but the response of the initial collecting segment is reduced in operated eels. The increase of the nuclear area in the various epithelia is no longer discernable, only some nucleolar hypertrophy may be detected. Some mitotic activity is present in the epithelia, and numerous “buds” (compact cell masses) appear, close to the collecting segment, which differentiate gradually in new kidney tubules. This process is similar to that already described in intact eels treated with prolactin for 10 days. These modifications may indicate a stimulation of the kidney. Some comments are made on the lymphoid tissue close to the kidney, and on the juxtaglomerular cells. In hypophysectomized eels, prolactin still acts on the kidney, although its effect is not as marked as in intact eels. Despite the fact that freshwater survival of hypophysectomized eels is excellent some hypophysial factor (s), in addition to the fish prolactin-like hormone, may be required to allow a full response to prolactin. These histological data are discussed in the light of previously published results on sodium fluxes, and blood and urine electrolyte levels, in intact and hypophysectomized teleosts, and after a prolactin treatment in freshwater.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 88 (1968), S. 576-590 
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé La structure histologique du rein de l'Anguille (étudiée après diverses colorations) répond aux descriptions données pour les reins des Téléostéens d'eau douce. Le néphron comprend un glomérule, un collet cilié, auquel font suite les divers segments du néphron: proximal, formé lui-même de deux parties distinctes, distal, collecteur initial et collecteur général. Un traitement avec la prolactine ovine (24 U.I. par jour) entraîne des modifications importantes de la structure histologique du rein, qui semblent maximales après une dizaine d'injections. Les modifications les plus nettes atteignent le segment collecteur initial: de diamètre accru, il présente un cytoplasme clair, des noyaux et des nucléoles hypertrophiés, une membrane nucléaire plus chromophile. Les mesures de la hauteur cellulaire des différentes parties du tubule, de la largeur du tubule et de sa lumière ainsi que des diamètres nucléaires transversaux mettent en évidence une augmentation statistiquement significative. De nombreuses mitoses apparaissent dans tous les segments du tubule, dont l'aspect général suggère l'idée d'une stimulation. En outre, de très nombreux bourgeons naissent au contact du collecteur initial. Leur différenciation conduit à la formation de nouveaux tubules rénaux, bientót fonctionnels. Ces données histologiques sont envisagées en corrélation avec les changements des métabolismes de l'eau et des electrolytes observés chez divers Téléostéens lors de traitements avec de la prolactine.
    Notes: Summary The histological structure of the kidney of the Atlantic eel is described. It appears typical of that of a freshwater teleost: the nephron includes a glomerulus, a ciliated neck segment, a proximal segment with a brush border which can be subdivided into 2 distinct portions, a distal segment, an initial collecting segment and a general collector. Injections of ovin-prolactin (24 U.I. per day) in the body cavity induce important modifications of the kidney structure. The most visible changes affect the initial collecting segment: its diameter increases, cells exhibit a clear cytoplasma, hypertrophied nuclei and nucleoli, with a chromatic nuclear membrane. In the various segments, the measurements of the cell height, of the width of the tubule and its lumen, and of the nuclear diameter show an increase which is statistically significant. A mitotic activity occurs in all segments of the tubule. These changes which appear maximal after 10 injections, suggest a stimulation by a prolactin treatment. In addition, numerous “buds” (or compact cell masses) appear close to the initial collecting segment; their differentiation leads to the formation of new kidney tubules which become rapidly active. These histological data are discussed in correlation with the modifications of water and electrolyte metabolism in various teleosts, produced by prolactin administration.
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  • 7
    Publication Date: 1969-01-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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  • 8
    Publication Date: 1968-01-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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  • 9
    Publication Date: 2020-06-03
    Description: SUMMARY On 10 May 2018, an unprecedented long and intense seismic crisis started offshore, east of Mayotte, the easternmost of the Comoros volcanic islands. The population felt hundreds of events. Over the course of 1 yr, 32 earthquakes with magnitude greater than 5 occurred, including the largest event ever recorded in the Comoros (Mw = 5.9 on 15 May 2018). Earthquakes are clustered in space and time. Unusual intense long lasting monochromatic very long period events were also registered. From early July 2018, Global Navigation Satellite System (GNSS) stations and Interferometric Synthetic Aperture Radar (InSAR) registered a large drift, testimony of a large offshore deflation. We describe the onset and the evolution of a large magmatic event thanks to the analysis of the seismicity from the initiation of the crisis through its first year, compared to the ground deformation observation (GNSS and InSAR) and modelling. We discriminate and characterize the initial fracturing phase, the phase of magma intrusion and dyke propagation from depth to the subsurface, and the eruptive phase that starts on 3 July 2018, around 50 d after the first seismic events. The eruption is not terminated 2 yr after its initiation, with the persistence of an unusual seismicity, whose pattern has been similar since summer 2018, including episodic very low frequency events presenting a harmonic oscillation with a period of ∼16 s. From July 2018, the whole Mayotte Island drifted eastward and downward at a slightly increasing rate until reaching a peak in late 2018. At the apex, the mean deformation rate was 224 mm yr−1 eastward and 186 mm yr−1 downward. During 2019, the deformation smoothly decreased and in January 2020, it was less than 20 per cent of its peak value. A deflation model of a magma reservoir buried in a homogenous half space fits well the data. The modelled reservoir is located 45 ± 5 km east of Mayotte, at a depth of 28 ± 3 km and the inferred magma extraction at the apex was ∼94 m3 s−1. The introduction of a small secondary source located beneath Mayotte Island at the same depth as the main one improves the fit by 20 per cent. While the rate of the main source drops by a factor of 5 during 2019, the rate of the secondary source remains stable. This might be a clue of the occurrence of relaxation at depth that may continue for some time after the end of the eruption. According to our model, the total volume extracted from the deep reservoir was ∼2.65 km3 in January 2020. This is the largest offshore volcanic event ever quantitatively documented. This seismo-volcanic crisis is consistent with the trans-tensional regime along Comoros archipelago.
    Print ISSN: 0956-540X
    Electronic ISSN: 1365-246X
    Topics: Geosciences
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  • 10
    Publication Date: 2013-09-01
    Print ISSN: 2150-704X
    Electronic ISSN: 2150-7058
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Published by Taylor & Francis
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