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  • 1
    Electronic Resource
    Electronic Resource
    Mineral and Organic Matrix Interaction in Normally Calcifying Tendon Visualized in Three Dimensions by High-Voltage Electron Microscopic Tomography and Graphic Image Reconstruction
    Amsterdam : Elsevier
    Journal of Structural Biology 110 (1993), S. 39-54 
    Details
    ISSN: 1047-8477
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/jsbi.1993.1003
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  • 2
    Electronic Resource
    Electronic Resource
    The Death of an Amoeba (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A geneological study shows that about 4% of the cells in healthy, adequately fed Amoeba proteus cultures are inviable. Two different categories of inviability are distinguished. About 44% of all inviability involves twin sisters formed at a division. Another 39% involves single cells with viable sisters and nieces. The inviable singles usually die more rapidly and show fewer visible abnormalities than the twins. The mothers of inviable twins show an increased interdivision time compared to mothers of inviable singles. Both categories of death are more rapid than starvation. The 17% of the deaths which involve aunt-niece pairs appear to be special cases of twin sister or single cell deaths. There is no evidence for stem line division where a cell forms only one viable daughter for several generations. It is proposed that death is a normal occurrence in amoeba populations. It occurs regardless of culture conditions and may be a measure of accumulated lethal mutations in an asexual polyploid organism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb04313.x
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  • 3
    Electronic Resource
    Electronic Resource
    Partial Purification of a Species-Specific Lethal Factor from Amoeba indica (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The species-specific lethal factor obtained from Amoeba indica, a freeliving mononucleate amoeba, can be partially purified by differential centrifugation, gel exclusion chromatography, and elution from Cibacron blue-agarose. The factor is unstable in the partially purified form, but activity is correlated with a heat-labile protein having an apparent molecular weight of 186,000. The factor causes inhibition of division and death when injected into A. proteus. Purification of the lethal factor indicates that aggregation of the factor with amoebal proteins is not a hindrance to further investigation. The natural role of the factor is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02816.x
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  • 4
    Electronic Resource
    Electronic Resource
    Variability of the limited lifespan state in ameba
    Amsterdam : Elsevier
    Experimental Cell Research 127 (1980), S. 261-267 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(80)90431-0
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of limited life span in small clones
    Amsterdam : Elsevier
    Journal of Theoretical Biology 71 (1978), S. 453-464 
    Details
    ISSN: 0022-5193
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0022-5193(78)90172-8
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  • 6
    Electronic Resource
    Electronic Resource
    Uptake of cyanobacteria contained in oil drops by plant protoplasts (1979)
    Springer
    Naturwissenschaften 66 (1979), S. 111-112 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00373506
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  • 7
    Electronic Resource
    Electronic Resource
    Demonstration of ricin within the mammalian para-aortic lymph node (1989)
    Springer
    Journal of molecular histology 21 (1989), S. 380-386 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Following a supralethal injection of ricin into thigh muscle of the adult rat, the toxin was demonstrated post-mortem in the para-aortic lymph node, ipsilateral to the side of injection. The relative merits of two immunoenzyme methods, peroxidase anti-peroxidase (PAP) and avidin—biotin—peroxidase complex (ABC) and a silver-enhanced immunogold method (IGSS) were assessed in the detection of ricin in the lymph node tissue. The toxin was clearly seen to be located in association with histiocytes found both within and lining the sinuses of the nodes and also, in some cases, in the subcapsular sinus of the node; the toxin was not demonstrable within lymphoid follicles by light microscopy. However, using electron microscopy and the IGSS technique, cells carrying discrete particles of gold could be visualized within follicular areas. The IGSS and ABC-peroxidase methods were both found to give excellent results without background staining at the light microscopy level. However, when these techniques were used prior to embedding and viewing by electron microscopy, the IGSS technique proved to be far superior.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01789735
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  • 8
    Electronic Resource
    Electronic Resource
    Distribution of ricin within the mammalian para-aortic lymph node (1989)
    Springer
    Journal of molecular histology 21 (1989), S. 387-392 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous work has shown that, following an intramuscular injection of ricin, the toxin becomes localized within histiocytes in the sinuses of lymph nodes draining the ‘wound’ site. When ricin labelled with colloidal gold was similarly injected, it was found within the same lymphoid cells as seen with native ricin. Biologically inert Indian ink apparently follows a similar fate, as demonstrated by the appearance of carbon particles within sinus histiocytes, as soon as 1 h after intramuscular injection. When the bindingin vitro of Indian ink or ricin toxin to sections of lymph node was examined, ricin was seen to bind to the surfaces of the same sinusoidal cells and also, with a much lower frequency, to follicular lymphocytes, whereas Indian ink failed to bind. This indicated an interaction between ricin and cell membrane components. Moreover, this binding was inhibited markedly by the galactose-containing disaccharide, lactose, a target sugar specified by the lectin binding site of ricin and to a much lesser extent by the monosaccharide mannose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01789736
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  • 9
    Electronic Resource
    Electronic Resource
    Confocal microscopy and three-dimensional reconstruction of electrophysiologically identified neurons in thick brain slices (1991)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 18 (1991), S. 11-23 
    Details
    ISSN: 0741-0581
    Keywords: Fluorescent microscopy ; Light microscopy ; Confocal light microscopy ; Neurobiology ; Hippocampus ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Three-dimensional morphology and electrophysiology were correlated from individual neurons in a thick brain slice preparation. The hippocampal formation from immature and adult rats was cut transverse to the longitudinal axis into 500 μ Um-thick slices which were maintained under physiologic conditions. Individual neurons were impaled and physiologically characterized using microelectrodes. Recordings were made from the soma and in some cases from a dendrite. The impaled neurons were filled through the microelectrode with the fluorescent dye lucifer yellow and imaged by confocal scanning laser microscopy using an analog preprocessor. As many as 180 optical sections were recorded as a function of depth through the slices. Images are presented as a series of optical sections, stereo pairs, or three-dimensional reconstructions. Both stereo contouring and volume rendering methods were employed, and the reconstructions were viewed from any arbitrary perspective. Dendritic and axonal fields were separated from each other and displayed separately or as different pseudocolors. The three-dimensional reconstructions provided perspectives that were difficult or impossible to appreciate by viewing the optical sections or conventionally formed stereo pairs.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060180104
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  • 10
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    Detection of limited life span in small clones (1978)
    Elsevier
    Details
    Publication Date: 1978-04-01
    Print ISSN: 0022-5193
    Electronic ISSN: 1095-8541
    Topics: Biology
    Published by Elsevier
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