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  • 1
    Electronic Resource
    Electronic Resource
    Production of Poly(β-Hydroxybutyric Acid) by Recombinant Escherichia colia (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 721 (1994), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1994.tb47375.x
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  • 2
    Electronic Resource
    Electronic Resource
    Characteristics of Poly(3-Hydroxybutyric Acid) Synthesis by Recombinant Escherichia coli (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 782 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb40555.x
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  • 3
    Electronic Resource
    Electronic Resource
    Deciphering bioplastic production (2006)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 24 (2006), S. 1227-1229 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The shift toward sustainable production systems has focused attention on bioplastics, which can be produced by many microorganisms from renewable raw materials. Polyhydroxyalkanoates (PHAs) are natural polyesters synthesized in the form of discrete intracellular granules in abundances that can ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1006-1227
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  • 4
    Electronic Resource
    Electronic Resource
    The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 1275-1281 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The rumen represents the first section of a ruminant animal's stomach, where feed is collected and mixed with microorganisms for initial digestion. The major gas produced in the rumen is CO2 (65.5 mol%), yet the metabolic characteristics of capnophilic (CO2-loving) microorganisms are not well ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1010
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  • 5
    Electronic Resource
    Electronic Resource
    Development of DNA microarray for the monitoring of Escherichia coli metabolism (1999)
    [s.l.] : Nature America, Inc.
    Nature genetics 23 (1999), S. 56-56 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] We developed a microarrayer system for the manufacture of DNA chips. The 3-axis robot was designed to automatically collect samples from 96- or 384-well microtiter plates using up to 16 pens simultaneously and deposit them on a modified glass slides. This is followed by a wash/dry operation and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/14345
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  • 6
    Electronic Resource
    Electronic Resource
    E. coli moves into the plastic age (1997)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 15 (1997), S. 17-18 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Over the past 25 years, petrochemical-derived plastics have become indispensable materials in modern life. These plastics,however, are not biodegradable and cause significant waste disposal and contamination problems. Increasing awareness of the harmful effects of nonbiodegradable plastics on the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0197-17b
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  • 7
    Electronic Resource
    Electronic Resource
    Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 214 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The Escherichia coli fabGEc gene and the Pseudomonas aeruginosa rhlGPa gene, which encode 3-ketoacyl-acyl carrier protein reductase, were expressed in E. coli W3110 and its fadA mutant strain WA101 to examine their roles in medium-chain-length (MCL) polyhydroxyalkanoate (PHA) biosynthesis from fatty acids. When one of these 3-ketoacyl-acyl carrier protein reductase genes was co-expressed with the Pseudomonas sp. 61–3 PHA synthase gene (phaC2Ps) in E. coli W3110, MCL-PHA composed mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate was synthesized from sodium decanoate. When the fabGEc gene and the phaC2Ps gene were co-expressed in the fadA mutant E. coli strain WA101, MCL-PHA rich in 3-hydroxydecanoate monomer up to 93 mol% was accumulated from sodium decanoate. This was possible by efficiently redirecting 3-ketoacyl-coenzymes A from the β-oxidation pathway to the PHA biosynthesis pathway without losing two carbon units, the strategy of which can be extended for the production of MCL-PHAs rich in other specific monomers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11350.x
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  • 8
    Electronic Resource
    Electronic Resource
    Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824 (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 108 (1993), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The copy number and stability of several plasmid vectors in Clostridium acetobutylicum ATCC 824 were determined. The protocols were modified from the traditional ones to overcome the problems associated with unusual behavior of C. acetobutylicum cells on solid medium. The plasmid copy numbers of pSYL2, pFNK1, pFNK3, and pFNK5 in strain ATCC 824 were 14, 8, 6, and 6, respectively. pSYL2 and pFNK1 were segregationally stable, since the fractions of plasmid-carrying cells after 60 generations of growth without antibiotic (erythromycin) were 73% and 77%, respectively. Vector pFNK1 carrying fermentative genes was found to be rather unstable. The observed instability seemed to be due to the complex host-plasmid interactions by amplified expression of enzymes involved in the tightly regulated primary metabolism of C. acetobutylicum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06122.x
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  • 9
    Electronic Resource
    Electronic Resource
    Enhanced production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinantEscherichia coli in a defined medium (1996)
    Springer
    Journal of polymers and the environment 4 (1996), S. 131-134 
    Details
    ISSN: 1572-8900
    Keywords: Escherichia coli ; poly(3-hydroxybutyrate) (PHB) ; filamentation ; fed-batch culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Fed-batch cultures of recombinantEscherichia coli strains were carried out for the production of poly(3-hydroxybutyric acid) (PHB) in a chemically defined medium. TheE. coli strains used were XL1-Blue, harboring pSYL105, a stable high-copy number plasmid containing theAlcaligenes eutrophus polyhydroxyalkanoate (PHA) genes, and XL1-Blue, harboring pSYL107, which is pSYL105 containing theE. coli ftsZ gene to suppress filamentation. With XL1-Blue(pSYL105) the final cell mass and PHB concentration obtained in 62 h were 102 and 22.5 g/L, respectively. Fed-batch culture of XL1-Blue(pSYL107) under identical conditions resulted in a final cell mass and PHB concentration of 127.5 and 48.2 g/L, respectively. The PHB contents obtained with XL1-Blue(pSYL105) and XL1-Blue(pSYL107) were 22.1 and 37.8%, respectively. Therefore, PHB was more efficiently produced in a defined medium by employing filamentation-suppressed recombinantE. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02074874
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of complex nitrogen source on the synthesis and accumulation of poly(3-hydroxybutyric acid) by recombinantEscherichia coli in flask and fed-batch cultures (1994)
    Springer
    Journal of polymers and the environment 2 (1994), S. 169-176 
    Details
    ISSN: 1572-8900
    Keywords: Escherichia coli ; poly(3-hydroxybutyric acid) ; defined medium ; complex nitrogen source ; fed-batch culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract When a recombinantEscherichia coli XL1-Blue harboring pSYL105 was cultured in a complex medium, a poly(3-hydroxybutyric acid) (PHB) concentration of 7.16 g/L was obtained in 48 h. However, a PHB concentration of only 0.91 g/L was obtained in 60 h by culturing in a defined medium. Also, fed-batch culture in a defined medium resulted in considerably lower PHB accumulation than in a complex medium. With the aim to produce a high concentration of PHB at a reduced medium cost, we examined 10 complex nitrogen sources for their ability to promote PHB synthesis in a defined medium. Tryptone, casamino acids, and casein hydrolysate promoted PHB synthesis to a higher extent than the others tested. PHB synthesis was also enhanced during fedbatch cultures when a defined medium was supplemented with various complex nitrogen sources. With tryptone supplementation a PHB concentration of 66.7 g/L could be obtained in 44 h. Yeast extract was less effective for promoting PHB synthesis than tryptone. Corn steep liquor, which did not enhance PHB synthesis significantly, could promote PHB synthesis considerably when supplemented together with yeast extract in both flask and fed-batch cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02067442
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