ISSN:
1432-203X
Keywords:
Key words: Plant cells – Fresh weight – Dry weight – Microcentrifuge tube
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary. A simple method using microcentrifuge tubes for determining fresh and dry weights, and collecting cell-free supernatant from plant suspension cultures is described. This method offers improvements in accuracy, precision, and time efficiency over traditional filtration methods. Using 4-day-old Nicotinia tabacum cultures, the centrifuge method was shown to remove 25% more of the interstitial water from cell aggregates compared to a suction filtration method, with significantly less variation in fresh weight data.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050088
Permalink