ISSN:
1432-1203
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Analysis of amplified polymerase chain reaction products of 575 bp from the fourth exon of the human type I 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase gene at locus HSDβ3 1p11–p13, reveals a frequent two-allele polymorphism at codon Leu338 due to a silent substitution of T by C, thus creating a BglII site leading to 371- and 204-bp fragments. Southern blot analysis of BglII-digested DNA from 57 individuals using a genomic probe detects two allelic fragments of 5.3kb and 0.77 kb, respectively, while two allelic fragments of 3.7 kb and 3.4 kb are obtained in TaqI digests with multiple constant bands, as also observed with BglII digests.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00201743
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