Publication Date:
1998-08-26
Description:
Nitrogen-fixing bacteroids in legume root nodules are surrounded by the plant-derived peribacteroid membrane, which controls nutrient transfer between the symbionts. A nodule complementary DNA (GmSAT1) encoding an ammonium transporter has been isolated from soybean. GmSAT1 is preferentially transcribed in nodules and immunoblotting indicates that GmSAT1 is located on the peribacteroid membrane. [14C]methylammonium uptake and patch-clamp analysis of yeast expressing GmSAT1 demonstrated that it shares properties with a soybean peribacteroid membrane NH4〈SUP ARRANGE="STAGGER"〉+ channel described elsewhere. GmSAT1 is likely to be involved in the transfer of fixed nitrogen from the bacteroid to the host.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, B N -- Finnegan, P M -- Tyerman, S D -- Whitehead, L F -- Bergersen, F J -- Day, D A -- Udvardi, M K -- New York, N.Y. -- Science. 1998 Aug 21;281(5380):1202-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9712587" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Base Sequence
;
Biological Transport
;
Carrier Proteins/chemistry/*genetics/*metabolism/*secretion
;
*Cation Transport Proteins
;
Cell Membrane/metabolism
;
DNA, Complementary
;
Ion Channels/metabolism
;
Kinetics
;
Methylamines/metabolism
;
Molecular Sequence Data
;
Organelles/metabolism
;
Patch-Clamp Techniques
;
Plant Roots/genetics/metabolism/microbiology
;
Potassium/metabolism
;
Quaternary Ammonium Compounds/*metabolism
;
Saccharomyces cerevisiae/genetics/metabolism
;
*Soybean Proteins
;
Soybeans/chemistry/*genetics/metabolism/microbiology
;
Spheroplasts/metabolism
;
Symbiosis
;
Transformation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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