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  • 1
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Young trees of European beech (Fagus sylvatica L.) were exposed in a phytotron to different levels of ozone and CO2 under the climatic simulation of one vegetation period. High ozone levels were simulated similar to high ozone concentration in the field (up to 110 ppb), while CO2 was added as 300 ppm to the present level of ca 380 ppm. Our study describes different aspects of photosynthesis from the leaf level to the reactions of selected thylakoid components at different harvest times during growth of the beech trees under the different fumigation regimes. Ozone effects appeared in the first weeks of the treatment as a stimulation of chlorophyll fluorescence (Fv/Fm), in oxygen production and in ribulose-1,5-bisphosphate carboxylase/oxygenase activity, while the summer and early autumn harvests showed strong reductions in these parameters. Only phosphoenolpyruvate carboxylase (PEPcase) activity remained higher under high ozone. The effects of high CO2 appeared in general as a small stimulation in enzyme activity like PEPcase in spring. However, with increasing time of fumigation, reductions of all parameters were observed. Especially chlorophylls showed strong reductions under high CO2. The combined treatment with high ozone plus high CO2 resulted mostly in an amelioration of the negative ozone effects, although control levels were not reached.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 60 (1984), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A growth period from day 4 to day 11 after sowing of dark-grown oat seedlings (Avena sativa L. cv. Flämingskrone) was studied. Etioplasts were prepared from various stages. By means of one and two dimensional gel eletrophoresis developmental changes in the polypeptide pattern of the prolamellar body-prothylakoid complex of etioplasts were studied. Polypeptides were described by their molecular weights and pI values after isoelectric focusing. The polypeptide map changes continuously with age, with the onset of effects of senescence around day 7. Concomitantly, proto-chlorophyllide and proteins are degraded, with a higher degradation rate for proto-chlorophyllide. An analysis of protochlorophyllide fluorescence displayed a constant ratio (fluorescence emission at 650 nm/fluorescence emission at 630 nm) from day 6 to day 11. It is concluded, that the best stages for greening studies of dark-grown oat seedlings occur between day 4 and day 6 after sowing.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Etioplasts of dark-grownAvena sativa plants were used to prepare either saponin-free or saponin-containing prolamellar bodies. Lipid extracts from both fractions were studied in reaggregation experiments: extracts containing saponins showed liposomes as well as tubules, while saponin-free samples formed only liposomes. Purified PLB lipids in reaggregation experiments were either studied in the presence or in the absence of saponins. Best tubule formation was found with samples containing MGDG+saponin. However, the reconstruction of PLB-like structures was not possible. The long tubules, protruding from isolated PLBs, are seen as a result of the reaction of saponins (originally located in vacuoles) with MGDG.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2285
    Keywords: Picea abies ; Cytogenetic bioindication ; Chromosomal aberrations ; Soil ; Ozone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The classification of chromosomal aberrations was used to characterize different factors affecting chromosomes in the root meristem of Norway spruce [Picea abies (L.) Karst.] trees. It is important to know the most significant factor affecting the chromosomes in the root meristem of plants at natural sites. The results suggest that an intensive site effect is more significant than the soil or the provenance of the individual. This cytogenetic plant test system was also used to investigate 5-year-old spruce trees exposed in environmental chambers to elevated concentrations of carbon dioxide (750 cm3m−3) and ozone (0.08 cm3m−3) as single variables or in combination, and then transferred to a field for observation of a “memory effect”. The fumigated variants showed an increased number of chromosomal aberrations compared to the controls, which carried on as a memory effect in the root meristems far beyond the fumigation period.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5052
    Keywords: Algae ; Chloroplast ; Micrasterias ; Photosynthesis ; Ultrastructure ; UV-B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exposure of postmitotic growing and non-growing cells of the unicellular green alga Micrasterias denticulata to different UV-B cut-off wavelengths together with simulated sunlight in a sun simulator has revealed a marked resistence of the algae against strong irradiation. While down to a cut-off wavelength of 284 nm irradiated during the most sensitive stage of cell development chloroplast ultrastructure remains unaffected, severe changes in arrangement and structure of stroma and grana thylakoids occur only at the lowest cut-off wavelengths of 280 and 275 nm. The structural alterations end up in a more or less complete desintegration of grana and stroma thylakoids with the remaining membraneous structures appearing in negative staining thus indicating drastic changes in membrane composition. Photosynthetic activity determined by chlorophyll fluorescence (ratio of variable to maximal fluorescence) and oxygen evolution responded more sensitively to UV-B irradiation. With decreasing UV cut-off wavelengths and prolonged incubation a decrease of photochemistry of PS II occured reaching its lowest values after 60 min at 275 and 280 nm. Oxygen production was even maintained under strong UV irradiation with a cut-off wavelenght of 275 nm up to 15 min. With prolonged UV-B treatment any activity was lost. HPLC separations of pigments exhibited the appearance of break-down products (mainly derivatives of chl b and chl a) with decreasing cut-off wavelength and increasing exposure time. The xanthophyll cycle pigments seemed to be unaffected at least for an irradiation period of 60 to 90 min at low UV cut-offs. Possible mechanisms of UV stress avoidance or protection are discussed with regard to the varying altitudes of the natural habitats of the algae.
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  • 6
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    PANGAEA
    In:  Supplement to: Springer, Karin; Lütz, Cornelius; Lütz-Meindl, Ursula; Wendt, Angela; Bischof, Kai (2017): Hyposaline conditions affect UV susceptibility in the Arctic kelp Alaria esculenta (Phaeophyceae). Phycologia, 56(6), 675-685, https://doi.org/10.2216/16-122.1
    Publication Date: 2023-03-23
    Description: The kelp Alaria esculenta represents a key species in high Arctic marine fjord ecosystems. However, the European Arctic is currently experiencing extensive environmental change. Glacial fjord systems, such as Kongsfjorden (Spitsbergen, Svalbard), are subjected to rising temperature, increased freshwater inflow from glaciers and melting snow and a changing ultraviolet (UV) radiation regime related to stratospheric ozone depletion. Thus, in addition to natural seasonality, sessile organisms require acclimation in order to adapt to an environment in transition. We examined the physiological and ultrastructural responses of A. esculenta to the combined exposure to hyposalinity and UV radiation. Photosynthetic quantum yield slightly decreased during a low-salinity treatment of 7 d. Exposure to UV radiation also lowered quantum yield, but specimens previously treated with hyposalinity were significantly less susceptible to UV than nontreated individuals. Concomitant with a loss of chlorophyll during the hyposaline treatment, phlorotannin and antioxidant contents were maintained, and samples treated with low salinities exhibited higher UV-screening characteristics as demonstrated by significantly higher absorption ratios at 300/680 nm. Ultrastructural analyses revealed a treatment-dependent swelling of cell walls and accumulations of phlorotannin-containing vesicles. Our findings point to a strategy by which kelps apply a fast and cost-efficient redistribution of phlorotannins rather than increased synthesis as a general stress response to different environmental drivers in contrast to stress-specific responses. The notion that acclimation to one stressor (low salinity) reflects increased tolerance towards a second stressor (UV radiation) supports the concept of 'cross-acclimation' as established for higher plants but not yet for seaweeds.
    Keywords: Antioxidant capacity, in Trolox Equivalents; beta-Carotene; Chlorophyll a; Chlorophyll c1+c2; EXP; Experiment; Family; Fucoxanthin; Initial slope of rapid light curve; KongsfjordenOA; Light saturation point; Maximal electron transport rate; Maximum photochemical quantum yield of photosystem II; Phlorotannins; Ratio; Species; Treatment; Violaxanthin + Antheraxanthin + Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 96 data points
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  • 7
    Publication Date: 2023-05-12
    Keywords: Characteristic; Event label; Family; Growth rate; Location; Mt_Stuart; MULT; Multiple investigations; Species; Strain; Svalbard; Temperature, technical; United States
    Type: Dataset
    Format: text/tab-separated-values, 71 data points
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  • 8
    Publication Date: 2023-05-12
    Keywords: alpha-Carotene; alpha-Tocopherol; Antheraxanthin; beta-Carotene; Chlorophyll a; Chlorophyll b; Family; High Performance Liquid Chromatography (HPLC); Lutein; MULT; Multiple investigations; Neoxanthin; Species; Strain; Svalbard; Violaxanthin; Violaxanthin + Antheraxanthin + Zeaxanthin; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 98 data points
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  • 9
    Publication Date: 2023-05-12
    Keywords: Adonixanthin; alpha-Carotene; alpha-Tocopherol; Antheraxanthin; Astaxanthin; beta-Carotene; Canthaxanthin; Chlorophyll a; Chlorophyll b; Echinenone; Event label; Family; High Performance Liquid Chromatography (HPLC); Hydroxyechinenone; Lutein; Mt_Stuart; MULT; Multiple investigations; Neoxanthin; Species; Strain; Svalbard; United States; Violaxanthin; Violaxanthin + Antheraxanthin + Zeaxanthin; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 209 data points
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  • 10
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    PANGAEA
    In:  Supplement to: Leya, Thomas; Rahn, Andreas; Lütz, Cornelius; Ramias, Daniel (2009): Response of arctic snow and permafrost algae to high light and nitrogen stress by changes in pigment composition and applied aspects for biotechnology. FEMS Microbiology Ecology, 67(3), 432-443, https://doi.org/10.1111/j.1574-6941.2008.00641.x
    Publication Date: 2023-10-28
    Description: Ten algal strains from snow and permafrost substrates were tested for their ability to produce secondary carotenoids and a-tocopherol in response to high light and decreased nitrogen levels. The Culture Collection of Cryophilic Algae at Fraunhofer IBMT in Potsdam served as the bioresource for this study. Eight of the strains belong to the Chlorophyceae and two strains are affiliated to the Trebouxiophyceae. While under low light, all 10 strains produced the normal spectrum of primary pigments known to be present in Chlorophyta, only the eight chlorophyceaen strains were able to synthesize secondary carotenoids under stress conditions, namely canthaxanthin, echinenone and astaxanthin; seven of them were also able to synthesize minor amounts of adonixanthin and an unidentified hydroxyechinenone. The two trebouxiophyceaen species of Raphidonema exhibited an unusually high pool of primary xanthophyll cycle pigments, possibly serving as a buffering reservoir against excessive irradiation. They also proved to be good alpha-tocopherol producers, which might also support the deactivation of reactive oxygen species. This study showed that some strains might be interesting novel candidates for biotechnological applications. Cold-adapted, snow and permafrost algae might serve as valuable production strains still exhibiting acceptable growth rates during the cold season in temperate regions.
    Keywords: Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; SPP1158
    Type: Dataset
    Format: application/zip, 3 datasets
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