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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 449 (2007), S. 894-897 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The breakup of the supercontinent Gondwanaland into Africa, Antarctica, Australia and India about 140 million years ago, and consequently the opening of the Indian Ocean, is thought to have been caused by heating of the lithosphere from below by a large plume whose relicts are now the Marion, ...
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial & engineering chemistry research 34 (1995), S. 1440-1448 
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 3
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The non-enzymatic modification of proteins through the Maillard reaction plays an important role in the loss of seed viability during seed storage. In the present study we examined whether the Maillard reaction reduces the activities of scavenging enzymes in Vigna radiata (mung bean) seeds during storage. Seeds were stored under various conditions for different duration. Maillard products were monitored by measuring protein fluorescence, and the activities of glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POX) were determined. The accumulation of Maillard products in seed axes increased during storage with increasing moisture content and temperature, and was correlated with the decline in seed vigour. The activities of GR, CAT and APX decreased in proportion to the increase in Maillard products at all the moisture contents and temperatures tested. These enzymatic changes were also correlated with seed vigour. However, the activities of SOD and POX remained unchanged and appeared to be less sensitive to the Maillard reaction.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 69 (1987), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The production of ethylene and carbon dioxide by Pinus radiata D. Don cotyledons cultured on shoot-forming medium in sealed Erlenmeyer flasks was studied. To establish the role of these gases on morphogenesis three experimental approaches were used: (1) capping the flasks with serum caps on various days, and removing the serum caps at different stages of morphogenesis, (2) absorbing the gases by setting up traps singly and in combination during the various stages of differentiation and (3) incubating the cultures under continuous flow of constant gas mixtures. The results indicate that both ethylene and carbon dioxide, which build up during the first 10 to 15 days of culture, promote morphogenesis. Excessive accumulation after the initiation of buds causes some degree of dedifferentiation. When both the gases were eliminated from the flasks, bud formation and growth were inhibited. In the absence of oxygen, ethylene and carbon dioxide fail to influence growth and morphogenesis.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 76 (1989), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The metabolism of 14C-putrescine and the changes in the endogenous concentrations of putrescine, spermidine and spermine were studied when cotyledons of Pinus radiata D. Don were cultured under shoot-forming (SF, + N6-benzyladenine) and non-shoot-forming (NSF, - N6-benzyladenine) conditions. Differences in the total uptake of 14C-putrescine during a 2 h pulse feeding were not significant between the SF and NSF cotyledons except on day 3. The maximum uptake of label was on day 3 in the SF cotyledons, which released the highest amount of 14CO2 as well. 14C from the labeled putrescine was incorporated mainly into γ-aminobutyric acid, aspartate and glutamate. High performance liquid chromatography of the endogenous polyamines indicated that spermidine was the most predominant polyamine in the cultured cotyledons of radiata pine. Spermine increased by about 60% in the SF and 25% in the NSF cotyledons between days 0 and 3 of culture.
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  • 6
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of ethylene on in vitro plant regeneration from frond and rhizome expiants of Platycerium coronarium was investigated. Ethylene levels in the culture vessels increased with time, resulting in a decrease in the percentage of sporophytes produced. Addition of the ethylene action inhibitor silver thiosulfate resulted in an increase in the percentage of plants regenerated, indicating an inhibitory effect of ethylene on regeneration. However, the presence of 2,5-norbornadiene was not effective in reversing the effect of ethylene. Inhibitors of ethylene biosynthesis, such as cobalt chloride, salicylic acid, benzylisothiocyanate, and aminoethoxyvinylglycine, were also ineffective in increasing sporophyte regeneration. 1-Aminocyclopropane-1-carboxylic acid, the ethylene precursor, was ineffective in increasing the level of ethylene in the culture vessels. Therefore, the biosynthetic pathway of ethylene in the fern P. coronarium appears to be different from that of higher plants but similar to that of some other ferns.
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  • 7
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract High frequency, direct regeneration of shoots was induced in leaf cultures ofPaulownia tomentosa, P. fortunei x P. tomentosa andP. kawakamii. The optimum culture medium for the leaf explants derived from shoot cultures was Murashige-Skoog (MS) medium supplemented with 10 μM indole-3-acetic acid and 50 μM benzyladenine. Up to 40 shoots were obtained over a 4 month culture period from each leaf explant. Rooting occurred spontaneously in the shoots that were about 1 cm tall when subcultured on phytohormone-free MS medium. The plantlets could be transplanted successfully. Some of the transplantedP. tomentosa plantlets flowered in the greenhouse one year after transplanting. The protocol is suitable not only for rapid multiplication of the various species ofPaulownia, but also for analytical studies associated with adventitious shoot regeneration.
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  • 8
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Apogamous sporophytes were produced on Platycerium coronarium gametophytes cultured in the presence of indole-3-acetic acid (IAA). The percentage of apogamy as well as the total number of apogamous sporophytes produced per gametophyte clump were highest in the presence of 40 μM IAA. When ethylene was allowed to accumulate in the culture vessel in the presence of an optimum level of IAA, the percentage and total number of apogamous sporophyte production decreased significantly. Using light microscope and confocal laser scanning microscope we have shown that nuclear size can be used as a quick parameter to estimate the ploidy level of P. coronarium.
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  • 9
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. High frequency, direct regeneration of shoots was induced in leaf cultures of Paulownia tomentosa, P. fortunei × P. tomentosa and P. kawakamii. The optimum culture medium for the leaf explants derived from shoot cultures was Murashige-Skoog (MS) medium supplemented with 10 μM indole-3-acetic acid and 50 μM benzyladenine. Up to 40 shoots were obtained over a 4 month culture period from each leaf explant. Rooting occurred spontaneously in the shoots that were about 1 cm tall when subcultured on phytohormone-free MS medium. The plantlets could be transplanted successfully. Some of the transplanted P. tomentosa plantlets flowered in the greenhouse one year after transplanting. The protocol is suitable not only for rapid multiplication of the various species of Paulownia, but also for analytical studies associated with adventitious shoot regeneration.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 25 (1991), S. 53-60 
    ISSN: 1573-5044
    Keywords: carbon dioxide ; ethylene ; gastight cultures ; Picea glauca ; plastic sealant ; serum-capping ; somatic embryos ; tissue viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effeet of long-term storage on the viability and regeneration eapacity of somatic embryogenic white spruce tissue (Picea glauca) was investigated. It was found that by keeping white spruce embryogenic tissue in serum-capped flasks at ambient temperatures, a viability of 80% could be maintained without subculturing for a period of over one year. Growth characteristics of the embryogenic tissue on solid medium and suspension cultures derived therefrom were essentially identical to the culture from which they originated. Ethylene and carbon dioxide accumulation peaked around days 8 to 10 in serum-capped cultures and declined thereafter during the short-term (20 days). When ethylene and carbon dioxide levels were measured over a period of five months, in flasks varying in their degree of gas tightness, it was found that those cultures that maintained higher carbon dioxide concentrations after five months remained viable and could be recultured under normal conditions. Development to cotyledon stage embryos from immature embryos could be obtained by exposure of the long-term storage material to 10 μM ABA, and the number of maturing embryos was found to be similar to that of the original culture.
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