ISSN:
0148-7280
Keywords:
nucleolar structure
;
RNA metabolism
;
oocyte
;
human
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
Nucleolar ultrastrure was studied in fully grown human oocytes obtained from multilaminar preantral follicles and from follicles at different stages of antrum formation. Selective staining for ribonucleoproteins and 3H-uridine labeling were used in attempt to better understand the nature and functional significance of homogeneous dense nucleoli found in oocytes from large antral follicles.There was an apparent increase in the radio of nucleonema to nucleolar interstices, accompanied by a gradual degranulation of the nucleolonema during early stages of antrum fromation. The process of nucleolar homogenization continued in oocytes from medium-size antralfollicles, island of more tightly packed fibrils being hybothesized to represent persisting active transcription units. Entirely filamentous and homogeneous nucleoli were typical for oocytes from large antral follicles. They were demonstrated to ribonucleoprotein filaments embedded in pale- staining matrix. They were demonstrated to contain newly synthesized RNA after a 30-min pulse with 3H-uridine. The described nucleolar transformations are interpreted as acorrelate of nucleolar transition from a site of RNA synthesis into a site of RNA Storage during in human oocyte preovulatory development.
Additional Material:
11 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1120080307
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