ISSN:
1572-9818
Keywords:
BAC
;
BAC-end sequencing
;
DNA isolation
;
sorghum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract With the aim of constructing a physical map of sorghum, we developed a rapid, high throughput approach for isolating BAC DNA suitable for restriction endonuclease digestion fingerprinting, PCR- based STS-content mapping, and BAC-end sequencing. The system utilizes a programmable 96 channel liquid handling system and associated accessories that permit bacterial cultivation and DNA isolation in 96-well plate format. This protocol details culture conditions that optimize bacterial growth in deep-well plates and criteria for BAC DNA isolation to obtain high yields of quality BAC DNA. The system is robust, accurate, and relatively cost-effective. The BAC DNA isolation system has been tested during efforts to construct a physical map of sorghum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007530620792
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