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  • 1
    Electronic Resource
    Electronic Resource
    Biotechnological aspects of the production of human pro-kallikrein using the AcNPV-baculovirus-expression system (1993)
    Springer
    Cytotechnology 11 (1993), S. 67-75 
    Details
    ISSN: 1573-0778
    Keywords: baculovirus-expression system ; human pro-kallikrein ; batch- and perfusion fermentation ; media comparison ; process development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We have investigated large scale production processes (up to 2 liters) of recombinant proteins using the baculovirus expression system in order to optimize the product yields. Experiments using cell lines ofSpodoptera frugiperda (Sf9) andMamestra brassicae (IZD-Mb0503) were performed to show the different production capacities of the cell lines. The influence of the infection at different cell densities is described. Beyond that, TC100-, IPL41- and serum-free IPL41-medium were compared to demonstrate their different capabilities of supporting cell growth and protein expression. Additionally, the inhibitory effect of FCS on the protease activity of kallikrein, which is produced in its zymogenic form, is discussed. Improved production parameters are described, which enabled us to produce up to 8000 units of activated pro-kallikrein within 14 days using perfusion cultivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749059
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  • 2
    Electronic Resource
    Electronic Resource
    Multistage production of Autographa californica nuclear polyhedrosis virus in insect cell cultures (1990)
    Springer
    Cytotechnology 4 (1990), S. 271-278 
    Details
    ISSN: 1573-0778
    Keywords: baculovirus ; in vitro production ; perfusion cultivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The aim of our study was to establish an efficient system for thein vitro production of the insect pathogenic Autographa californica nuclear polyhedrosis virus in a Spodoptera frugiperda cell line. We optimized cultivation conditions for cell proliferation as well as for virus replication in a 1.5 litre stirred tank bioreactor. Cell and virus propagation were found to be optimal at a constant oxygen tension of 40%. In order to provide sufficient nutrients during virus synthesis filtration and perfusion devices were connected to the bioreactor. A virus production procedure in a repeated batch mode by using a two stage bioreactor system is described. Stage I was optimized for cell production and stage II for virus production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00563787
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    Paper (German National Licenses)
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