ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Synechococcus PCC 7942 was crystallized in two different forms by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. Form I crystals were hexagonal, space group P65 or P61, with unit-cell parameters a = b = 91.1, c = 428.6 Å, γ = 120°. Form II crystals were monoclinic, space group C2, with unit-cell parameters a = 152.3, b = 80.9, c = 213.6 Å, β = 103.1°. Native data were collected from a frozen crystal of form I to a resolution of 2.8 Å using synchrotron radiation at SPring-8, whereas form II crystals were easily damaged by radiation at room temperature and increased mosaicity in cryoprotectant solutions. A molecular-replacement solution of the form I crystal was obtained in space group P65 using the program AMoRe and the structure of the NAD-dependent GAPDH from Bacillus stearothermophilus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444901005285
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