ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 50 (1994), S. 429-440 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: In protein crystallography, the initial experimental problem is the identification of physical and chemical conditions that will support nucleation and crystal growth. Ideally, experiments to search for such conditions would be based on a full-factorial structure, with variation in the temperature and solution composition. However, consideration of even a moderate number of possibilities for the composition of the system will result in factorial experiments which may be prohibitively large. In this paper it is proposed that search experiments for protein crystallization might be based on orthogonal arrays. These are subsets of full-factorial experiments which possess a great deal of symmetry, such that a uniform distribution of points throughout the experimental region is preserved. Such experiments have reasonable size, explore the proposed experimental region in a systematic fashion, and form a logical basis for a sequential approach to the search for crystallization conditions. Examples of such initial search experiments are given, and their application to some recent protein crystallization problems in this laboratory is described briefly. The relationship of this approach to other protein crystallization search procedures is also discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 49 (1993), S. 331-343 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The structure of apo-azurin from Alcaligenes denitrificans has been determined at high resolution by X-ray crystallography. Two separate structure analyses have been carried out, (i) on crystals obtained from solutions of apo-azurin and (ii) on crystals obtained by removal of copper from previously formed crystals of holo-azurin. Data to 1.8 Å resolution were collected from the apo-azurin crystals, by Weissenberg photography (with image plates) using synchrotron radiation and by diffractometry, and the structure was refined by restrained least-squares methods to a final R value of 0.160 for all data in the range 10.0–1.8 Å. The final model of 1954 protein atoms, 246 water molecules (66 half-weighted), four SO42− ions, and two low-occupancy (0.13 and 0.15) Cu atoms has r.m.s. deviations of 0.012, 0.045 and 0.013 Å from standard bond lengths, angle distances and planar groups. For copper-removed azurin, data to 2.2 Å were collected by diffractometry and the structure refined by restrained least squares to a final R value of 0.158 for all data in the range 10.0–2.2 Å. The final model of 1954 protein atoms, 264 water molecules, two SO42− ions, two low occupancy (0.18 and 0.22) metal atoms and one unidentified atom (modelled as S) has r.m.s. deviations of 0.013, 0.047 and 0.012 Å from standard bond lengths, angle distances and planar groups. The two structures are essentially identical to each other and show no significant differences from the oxidized and reduced holo-azurin structures. The ligand side chains move slightly closer together following the removal of copper, with the radius of the cavity between the three strongly binding ligands, His 46, His 117 and Cys 112, shrinking from 1.31 Å in reduced azurin to 1.24 Å in oxidized azurin and 1.16 Å in apo-azurin. There is a suggestion of increased flexibility in one of the copper-binding loops but the structure supports the view that the copper site found in holo-azurin is a stable structure, defined by the constraints of the polypeptide structure even in the absence of a bound metal ion.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 185 (1960), S. 862-863 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To explore this further, small beams of f-in. square cross-section were cut from billets of three species, namely, Eucalyptus regnans (a hardwood prone to collapse of the cell wall during seasoning), Eucalyptus pilularis (a hardwood with very little tendency to collapse), and Pinus radiata (a ...
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Biochemistry 67 (1998), S. 545-579 
    ISSN: 0066-4154
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Chemistry and Pharmacology , Biology
    Notes: Abstract The nucleosome, which is the primary building block of chromatin, is not a static structure: It can adopt alternative conformations. Changes in solution conditions or changes in histone acetylation state cause nucleosomes and nucleosomal arrays to behave with altered biophysical properties. Distinct subpopulations of nucleosomes isolated from cells have chromatographic properties and nuclease sensitivity different from those of bulk nucleosomes. Recently, proteins that were initially identified as necessary for transcriptional regulation have been shown to alter nucleosomal structure. These proteins are found in three types of multiprotein complexes that can acetylate nucleosomes, deacetylate nucleosomes, or alter nucleosome structure in an ATP-dependent manner. The direct modification of nucleosome structure by these complexes is likely to play a central role in appropriate regulation of eukaryotic genes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1617-4623
    Keywords: Key words Heat shock factor ; Stress response ; Transcription Schizosaccharomyces pombe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heat shock factor (hsf) is the transcriptional activator that governs the transcriptional response of eukaryotic cells to stressful conditions. The structure and regulation of hsf is highly conserved. We describe deletion mutations in hsf+ that alter the ability of Schizosaccharomyces pombe to respond to different stressful conditions. One mutation causes increased sensitivity to cadmium while maintaining near normal sensitivity to heat stress, while another mutation confers increased sensitivity to heat stress but retains normal sensitivity to cadmium. Despite the differential sensitivity of these two strains to cadmium and heat stress, the mutant hsf proteins in each strain were activated by both cadmium and heat. However, we found that these mutations differentially affected the ability of hsf to activate different promoters: one mutated hsf activated the ssp1+ gene better than the wis2+ gene following either stress, while the other mutated hsf activated wis2+ better than ssp1+. We propose that the differential ability of strains that contain these mutant hsfs to survive cadmium and heat stress is not caused by differences in activation of hsf, but is caused instead by differential abilities of the mutant hsfs to activate the appropriate sets of genes needed for survival.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Elevated blood levels of deoxyguanosine in purine nucleoside phosphorylase deficient individuals have been implicated in T-cell immunodeficiency4, and we have previously shown that deoxyguanosine is toxic to proliferating embryonic thymic lymphocytes, but does not affect the epithelial framework of ...
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Wood science and technology 1 (1967), S. 81-98 
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Zusammenfassung Die von Preston und Kelsey entworfenen Modelle zur Darstellung der Längsschwindung von Holz wurden von den Verfassern erweitert und zwar im Hinblick auf Scherspannungen zwischen Mikrofibrillen und zwischen Fasern. Es wurden mathematische Beziehungen aufgestellt und gezeigt, daß früher entwickelte Modelle dem hier entworfenen angenähert entsprechen. sprechen. Die Eignung des Modells wird von verschiedenen Gesichtspunkten aus beleuchtet und die Anderungen der Modellparameter bei wechselndem Feuchtigkeitsgehalt werden erörtert. Der Einfluß des Querschneidens der Fasern beim Mikrotomschnitt, der Einfluß der Delignifizierung und das Auftreten einer anomalen Hysterese werden besprochen. Die Parameter der entwickelten Modelle sind als Kurven dargestellt; diese werden unter Berücksichtigung der in der ersten Mitteilung veröffentlichten Ergebnisse erörtert. Als Schlußfolgerung ergibt sich, daß die Nichtlinearität, die in der Beziehung zwischen Längsschwindung und Feuchtigkeitsgehalt beobachtet wurde auf die Entwicklung eines gewissen Widerstandes gegen das gegenseitige Gleiten der Kettenmoleküle zurückkzuführen ist, und daß dieser Widerstand durch eine Zunahme der zwischen den Ketten bestehenden Molekularverbindungen bei abnehmendem Feuchtigkeitsgehalt verursacht wird. Ebenso wird vorgeschlagen, die irreversible Scherverformung zwischen den Fibrillen als Ursache für die Hysterese anzusehen.
    Notes: Summary Models put forward by Preston and Kelsey to represent the longitudinal shrinkage of wood have been extended to take into account shearing stresses between microfibrils and between fibres. Mathematical relationships have been developed and previous models shown to be approximations to that developed here. The suitability of the model from various points of view and the variations in the model parameters with changing moisture content are discussed. The effect of the cross-cutting of the fibres occurring in thin microtomed sections, the effect of delignification and the incidence of an anomalous hysteresis are also discussed. Curves are given for the parameters of the models developed and these are discussed in the light of the results reported in Part I of this paper. It is concluded that the non-linearity observed in the longitudinal shrinkage moisture content relationship is due to the development of restraint against slipping of the chain molecules along one another and that this restraint is caused by increasing inter-chain molecular bonding with decreasing moisture content. It is also suggested that the irreversible interfibrillar shearing strain is a cause of the hysteresis.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary The incidence of non-linearity with stress in the rheological behaviour of wood in bending was shown earlier to occur at an unexpectedly low stress. This has been further confirmed by the study of an additional species, alpine ash. Tests on hoop pine, one of the species previously studied in bending, have shown that in compression, non-linearity first occurs at stresses which are a much higher fraction of the ultimate stress than in bending, but in approximately the same range of actual stress. This suggests that the cause of non-linearity at such a low stress in bending lies in the fact that the compressive strength is considerably lower than the bending strength. The stress on the compression face of a beam reaches a high percentage of the ultimate compressive strength by the time the bending stress reaches the limit of proportionality in bending and the nonlinear effects in a beam are attributable mainly to stresses near the compression face. A marked effect of temperature on fractional total creep for hoop pine in compression was found even within the range 20 to 50° C, the creep increasing by a factor of about 2.5 in this range.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Wood science and technology 6 (1972), S. 272-283 
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary Plastic stress arising in wood during drying is calculated according to the theoretical model developed earlier. The mechanism of stress reversal and the type of resudual stress corresponding to different values of material constants are shown. The results are in qualitative agreement with experimental evidence.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Wood science and technology 3 (1969), S. 324-328 
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Zusammenfassung Nach einer Begründung der Wichtigkeit der Rheologieforschung werden die hier verfolgten Einzelfragen kurz erläutert. Durch rheologische Untersuchungen kann zwar das voraussichtliche Verhalten des Holzes meist mit genügender Genauigkeit vorausgesagt werden, aber die Kenntnisse auf diesem Gebiet sind noch lückenhaft. Zu den wichtigsten Zusammenhängen gehört die Kenntnis der Wechselwirkungen zwischen Belastung einerseits und veränderlicher Holzfeuchte andererseits. Engere internationale Zusammenarbeit könnte hier, wie auch in anderen Fragen sehr nutzbringend sein.
    Notes: Summary The reasons for the interest in the rheology of wood and the favoured lines of research are stated. The response of wood can often be predicted adequately on the basis of previous studies but notable gaps remain in our knowledge. Future research should avoid going over well trodden ground and should concentrate on filling some of the gaps listed. The factor of greatest importance is the interaction of load with change in moisture content. International cooperation in rheological research on wood would be of great benefit.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...