ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary A cDNA clone, λLPO-1, encoding a major lignin peroxidase from the basidiomycete Bjerkandera adusta was isolated and characterized. The nucleotide sequence of λLPO-1 predicts a mature protein consisting of 349 amino acids with a molecular weight of 37,225 preceded by a signal peptide of 23 amino acid residues. We have also cloned and sequenced the gene encoding lignin peroxidase from B. adusta. Comparison of these sequences reveals a lignin peroxidase gene structure consisting of 1,116 bp of protein-encoding DNA that is interrupted by four intervening sequences. The putative eukaryotic regulatory sequence, a TATA box, is present at position — 75 relative to the translational initiation codon. Amino acid sequence homology between the coding regions of λLPO-1 and of the lignin peroxidase cDNA clone λML-1 from Phanerochaete chrysosporium is 61%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00169758
Permalink