Publication Date:
2019
Description:
Abstract
Klotho is a transmembrane protein known to regulate aging and lifespan. Soluble klotho (sKl), a truncated form of klotho, regulates various cell signaling pathways, including bone development. Here, we investigated the relationship between sKl and the zinc‐finger transcription factor early growth response protein 1 (EGR‐1) on bone formation. We find that sKl induces the expression of EGR‐1 mRNA and protein. Through mutational analysis, we identify the 130 bp region on the EGR‐1 promoter that is responsive to sKl overexpression. Additionally, sKl induces the expression of markers of bone differentiation (BMP2, RUNX2, ALP, COL1A, and Osteocalcin) in osteoblast MC3T3 cells. EGR‐1 siRNA decreases the bone mineralization induced by sKl or ascorbic acid/β‐glycerophosphate in MC3T3 cells. Our results suggest that sKl may regulate bone development through EGR‐1 expression.
Print ISSN:
0014-5793
Electronic ISSN:
1873-3468
Topics:
Biology
,
Chemistry and Pharmacology
Permalink