ISSN:
1432-203X
Keywords:
Abbreviations: MS, Murashige and Skoog; BAP, 6-benzylaminopurine; 2,4-D, 2,4-dichlorophenoxyacetic acid; NAA, 1-naphthaleneacetic acid; N6, Chu basal salt mixture; MES, 2-N-morpholinoethanesulfonic acid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Leaf mesophyll protoplasts of Dianthus superbus were cultured at a density of 5 × 104 protoplasts/ml and divided at about 18% plating efficiency in MS liquid medium supplemented with 0.5 mg/L BAP, 2.0 mg/L NAA and 9% mannitol after 2 weeks. Protocolonies formed after 3 to 4 weeks of culture in the dark at 27°C. These colonies were transferred to continuous illumination (21.5 μE m–2 sec–1 for 2 weeks where most of the colonies divided to form microcalli, about 2 mm in diameter. Subsequently, green microcalli were transferred to MS solidified medium with 2.0 mg/L 2,4-D that induced shoot-forming calli after 4 weeks. These calli were transferred onto N6-2 medium containing 0.1 mg/L 2,4-D, 0.1 mg/L NAA, 2.0 mg/L kinetin and 2.0 g/L casein hydrolysate and were cultured under light. After 5 weeks the calli gave rise to multiple shoots (10 to 15 per callus). Upon transfer to MS medium containing 2.0 mg/L NAA, individual shoots were rooted in 4 weeks. The regenerants were successfully transplanted into pointing soil.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050167
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