ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An investigation on the resolving power of hydroxyapatite columns is reported. The macromolecules chosen for this investigation have been five proteins endowed with a rigid structure and of different sizes: cytochrome c, lysozyme, β-lactoglobulin A, collagen, and T2 phage. The following points have been investigated in detail: (1) the dependence of the elution molarity upon column length and slope of the gradient; (2) the dependence of the elution molarity and the width of the protein peak upon the load and the presence of other chromatographic components; (3) the optimal conditions for the resolution of macromolecules having the same size or different sizes.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1970.360090303
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