ISSN:
1432-072X
Keywords:
2-Chloromaleylacetate
;
2,3-dichloromaleylacetate
;
Pseudomonas sp. strain B13
;
Maleylacetate reductase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleylacetate; 2 mol of NADH were consumed per mol of 2-chloro- and 2,3-dichloromaleylacetate while only 1 mol was necessary to catalyze the conversion of maleylacetate or 2-methylmaleylacetate. The maleylacetate reductase failed to use fumarylacetate as a substrate. The role of the enzyme in the chloroaromatics degradation is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00276301
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