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  • 1
    Keywords: perfused eel livers ; isolated eel hepatocytes ; cultured eel hepatocytes ; gluconeogenesis ; glycogen synthesis ; glucagon ; lipoprotein synthesis ; ganglioside GM4
    Description / Table of Contents: 1. Introduction --- 2. Eel as an experimental fish for studying liver functions --- 2-1. Availability of eel (Anguilla japonica) as an experimental fish --- 2-2. Perfusion of eel liver --- 2-3. Primary culture of eel hepatocytes --- 2-3A. Isolated eel hepatocytes --- 2-3B. Primary culture of eel hepatocytes --- 3. Glucose metabolisms in eel liver --- 3-1. Gluconeogenesis in rat liver --- 3-2. Gluconeogenesis in eel liver --- 3-2A. Gluconeogenesis by perfused eel liver --- 3-2B. Gluconeogenesis by isolated eel hepatocytes and cultured eel hepatocytes --- 3-3. Phosphoenolpyruvate synthesis pathway in eel liver --- 3-3A. Effects of inhibitors --- 3-3B. Subcellular distribution of enzymes --- 3-3C. Effects of leucine and other amino acids --- 3-3D. Effect of oleic acid --- 3-4. Comparison of PEP synthesis pathways between eel, rat, and pigeon liver --- 3-5. Glycogen metabolisms in eel liver --- 4. Lipoprotein metabolisms in eel liver --- 4-1. Characteristics of fish serum lipoproteins --- 4-2. Lipoproteins secreted by primary cultured eel hepatocytes --- 4-3. Effects of maturation on eel lipoprotein metabolism --- 4-3A. Comparison of body length, body weight, gonad-somatic index, and plasma thyroxine between silver and yellow eels --- 4-3B. Comparison of plasma lipoproteins between silver and yellow eels --- 4-3C. Comparison of lipoprotein synthesis by cultured hepatocytes of silver and yellow eels --- 4-3D. Effect of thyroxine on lipoprotein synthesis by cultured eel hepatocytes --- 4-4. HDL binding to primary cultured eel hepatocytes --- 4-4A. Stimulatory effect of HDL on VLDL-like lipoprotein synthesis and secretion --- 4-4B. ApoAI and apoAII of HDL do not function as a ligand for eel HDL receptor --- 4-4C. Ganglioside of HDL functions as a ligand for an HDL receptor of eel hepatocytes --- 4-4C-1. Ganglioside GM4 isolated from eel serum HDL --- 4-4C-2. GM4 as the ligand for eel HDL receptor --- 4-5. Vitellogenin induction by cultured eel hepatocytes --- 4-5A. Vitellogenin induction by estradiol-17β --- 4-5B. Vitellogenin induction by cultured eel hepatocytes --- 4-5C. Stimulatory effect of HDL on vitellogenin synthesis and secretion --- 5. Discussion --- 5-1. Integrity of a perfused eel liver, isolated and cultured hepatocytes --- 5-2. Gluconeogenesis and glycogen metabolisms in eel liver --- 5-2A. Gluconeogenesis --- 5-2B. Glycogen metabolisms --- 5-3. Lipoprotein metabolisms in eel liver --- 5-3A. Lipoprotein synthesized by cultured eel hepatocytes --- 5-3B. HDL metabolism --- 5-3C. Induction of vitellogenin synthesis by cultured eel hepatocytes. --- 5-4. General Discussion
    Pages: Online-Ressource (57 Seiten)
    ISBN: 1882322X
    Language: English
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  • 2
    Keywords: mitochondrion-rich cell ; chloride cell ; euryhalinity ; stenohalinity ; diadromous migration ; Mozambique tilapia ; killifish ; chum salmon ; Japanese eel ; fugu ; Japanese dace ; ion transport
    Description / Table of Contents: 1. Introduction --- 2. Mitochondrion-rich (MR) cells --- 2-1. General characteristics of MR cells --- 2-2. Molecular mechanisms of ion-transporting functions of MR cells --- 3. Euryhalinity and stenohalinity of teleosts --- 4. Mozambique tilapia --- 4-1. MR cells in the yolk-sac membrane of tilapia embryos and larvae --- 4-2. FW- and SW-type MR cells in tilapia embryos and larvae --- 4-3. Functions of multicellular complexes of SW-type MR cells --- 4-4. Functional differentiation of MR cells in the yolk-sac membrane --- 4-5. Functional classification of MR cells in the yolk-sac membrane --- 4-6. "Yolk ball" incubation system --- 4-7. Salinity tolerance of adult tilapia --- 4-8. Possible osmoreception by MR cells --- 5. Killifish --- 5-1. Transitional processes of MR-cell distribution during early life stages --- 5-2. Distinct FW- and SW-type MR cells --- 5-3. Functional alteration and replacement of MR cells --- 5-4. Ion-absorbing mechanisms of MR cells --- 6. Chum salmon --- 6.1. Hypoosmoregulatory ability of chum salmon embryo --- 6-2. Seawater adaptability in chum salmon fry --- 6-3. MR-cell turnover in the gills of chum salmon fry --- 6-4. Loss of hypoosmoregulatory ability in mature chum salmon --- 7. Japanese eel --- 7-1. Epidermal MR cells in embryos and larvae --- 7-2. Ontogenic changes in MR cells during leptocephalus and glass eel stages --- 7-3. MR cells in glass eel acclimated to FW --- 7-4. Gill MR cells in eel cultured in FW and those acclimated to SW --- 7-5. MR cells in yellow and silver eel --- 8. Fugu --- 8-1. Low-salinity tolerance of fugu --- 8-2. Gill MR cells in fugu --- 8-3. Functional significance of prolactin in a marine teleost of fugu --- 8-4. Comparison of growth in fugu reared in 25 and 100% SW. --- 9. Japanese dace --- 9-1. Acid tolerance of Osorezan dace --- 9-2. Molecular mechanisms of acid adaptation --- 10. Conclusions and future perspectives
    Pages: Online-Ressource (62 Seiten)
    ISBN: 1882322X
    Language: English
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  • 3
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: When a fresh specimen of yellowfin tuna Neothunnus albacora was analyzed, the cross-sectional area of ordinary (white) muscle fiber was 3.5 times larger than that of dark muscle. As heating temperature was raised, the shear force of dark muscle became progressively larger than that of ordinary muscle. Simultaneously, the A band in the ultrastructure of both muscles was stained more densely. While the Z line in I band of dark muscle still remained, that of ordinary muscle disappeared completely by heat treatment at 60°C for 30 min. Therefore, the differences in toughness change during heat treatment correlated well with that fiber sizes and ultrastructures of the two types of muscle.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Fish physiology and biochemistry 7 (1989), S. 337-342 
    ISSN: 1573-5168
    Keywords: parathyroid hormone (PTH) ; calcitonin ; calcitonin gene-related peptide (CGRP) ; hypocalcin ; radioimmunoassay ; immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunocytochemical localization of hypocalcin, a hypocalcemic factor in the corpuscles of Stannius (CS), in American eels was examined at the light (ABC method) and electron microscopic (protein A-gold technique) levels with the specific antiserum raised against purified rainbow trout hypocalcin. Only type 1 cells in the CS were immunoreactive in the light microscopic immunocytochemistry. At the electron microscopic level, however, hypocalcin immunoreactivity was observed in secretory granules of both type 1 and type 2 cells. Our findings may indicate that type 1 cells are the main source of hypocalcin, but that type 2 cells also produce it, suggesting that the presence of two cell types reflects different physiological conditions of a single cell type, rather than functionally different cell types. In addition, we summarize our recent data on the localization of other calcium regulatory, or putative calcium regulatory, hormones in fish: parathyroid hormone, calcitonin and calcitonin gene-related peptide.
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  • 5
    ISSN: 1573-5168
    Keywords: somatolactin ; chum salmon ; spawning migration ; gonadal development ; calcium ; energy metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plasma somatolactin (SL) concentrations were examined in chum salmon in relation to gonadal maturation; immature salmon in the Bering Sea at various stages of maturation, and mature salmon during upstream migration caught at the ocean, bay and river. Plasma SL concentrations as well as plasma prolactin (PRL) and growth hormone (GH) levels in the immature fish caught in the Bering Sea were maintained essentially at similar levels. Plasma SL in mature salmon increased significantly from the fish in the ocean to the fish in the river in both sexes. Although all the fish had fully developed gonads, females completed ovulation while still in the bay, whereas final spermeation in males was achieved after entry into the river. Thus, no clear correlation was seen between plasma SL levels and final gonadal maturation. On the other hand, plasma PRL concentrations in both male and female fish were higher in the fish in the river than those in the ocean and bay, and plasma GH levels were higher in both sexes in the fish in the bay and river than those in the ocean. Plasma levels of triglycerides, glucose, free fatty acids and ionized sodium and calcium were also examined. Significant-negative correlations were seen between plasma SL and plasma ionized calcium in mature male salmon, and between plasma SL and plasma triglycerides in mature female salmon. Although our findings do not rule out the possibility of the involvement of SL in final maturation, the results indicate that SL seems to be involved at least in energy and/or calcium metabolism during the spawning migration.
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  • 6
    ISSN: 1432-0878
    Keywords: Osteoclasts ; Bone resorption ; Acid phosphatase ; Capillary network ; Rainbow trout, Oncohynchus mykiss (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mineral-containing bone particles (BPs) were implanted intramuscularly into rainbow trout (Oncorhynchus mykiss) to investigate the sequence of appearance of bone-resorbing cells. A fibrous substance first surrounded the implanted BPs and was gradually replaced by connective tissue containing capillaries. Two weeks after BP implantation, relatively small multinucleated cells (type-1 cells), whose cytoplasm stained deeply with hematoxylin, appeared along the surfaces of the BPs. At later stages (after 4–8 weeks), the majority of cells which appeared to be resorbing the BPs were multinucleated cells whose cytoplasm stained deeply with eosin (type-2 cells). Type-2 cells contained more nuclei than type-1 cells. Electron-microscopical observations revealed that type-2 cells had the characteristic features of osteoclasts: the presence of numerous mitochondria, vacuoles and granules, and a differentiation of the cell membrane and cytoplasm into a ruffled border and clear zone, respectively. A tartrate-resistant acid phosphatase activity, which is an established characteristic of osteoclasts in terrestrial vertebrates, but which had not previously been examined in teleosts, was demonstrated histochemically in the type-2 cells. Development of type-2 cells was closely correlated with the development of connective tissue. These findings suggest that the development of a capillary network around the implanted BPs enables circulating osteoclast-progenitors to reach the surface of the BPs.
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  • 7
    ISSN: 1432-0878
    Keywords: Key words: Osteoclasts ; Bone resorption ; Acid phosphatase ; Capillary network ; Rainbow trout ; Oncorhynchusmykiss (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Mineral-containing bone particles (BPs) were implanted intramuscularly into rainbow trout (Oncorhynchus mykiss) to investigate the sequence of appearance of bone-resorbing cells. A fibrous substance first surrounded the implanted BPs and was gradually replaced by connective tissue containing capillaries. Two weeks after BP implantation, relatively small multinucleated cells (type-1 cells), whose cytoplasm stained deeply with hematoxylin, appeared along the s urfaces of the BPs. At later stages (after 4–8 weeks), the majority of cells which appeared to be resorbing the BPs were multinucleated cells whose cytoplasm stained deeply with eosin (type-2 cells). Type-2 cells contained more nuclei than type-1 cells. Electron-microscopical observations revealed that type-2 cells had the characteristic features of osteoclasts: the presence of numerous mitochondria, vacuoles and granules, and a differentiation of the cell membrane and cytoplasm into a ruffled border and clear zone, respectively. A tartrate-resistant acid phosphatase activity, which is an established characteristic of osteoclasts in terrestrial vertebrates, but which had not previously been examined in teleosts, was demonstrated histochemically in the type-2 cells. Development of type-2 cells was closely correlated with the development of connective tissue. These findings suggest that the development of a capillary network around the implanted BPs enables circulating osteoclast-progenitors to reach the surface of the BPs.
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  • 8
    ISSN: 1432-0878
    Keywords: Somatolactin ; Pituitary ; Gene expression ; In situ hybridization ; Immunocytochemistry ; Protein A-gold ; Oncorhynchus mykiss (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The gene expression and intracellular localization of somatolactin (SL), a putative pituitary hormone structurally related to both growth hormone and prolactin, were investigated in the pituitary of rainbow trout, Oncorhynchus mykiss. Using an in situ hybridization technique, we demonstrated the gene expression of the SL molecule in cells bordering the neurohypophysial tissue in the pars intermedia. These cells were identified immunocytochemically as SL-cells on the adjacent section. Electron-microscopic immunocytochemistry by means of the protein A-gold technique, also revealed that the SL-immunoreactivity was located mostly on the secretory granules in SL-cells. Our findings clearly indicate that SL is biosynthesized and stored in the granules in these cells.
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  • 9
    ISSN: 1432-0878
    Keywords: Key words: Chloride cell ; Embryo ; Larva ; Yolk-sac membrane ; Seawater adaptation ; Na+ ; K+-ATPase ; Tilapia ; Oreochromis mossambicus (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Morphological changes in the chloride cells (CCs) in the yolk-sac membrane of euryhaline tilapia (Oreochromis mossambicus) embryos and larvae were examined in relation to environmental salinity. Half of a brood of embryos spawned in fresh water (FW) were transferred directly to seawater (SW) 1 day before hatching; the other half was maintained in FW. The embryos and larvae in both FW and SW contained a rich population of CCs in the yolk-sac membrane; the CCs were visualized by whole-mount immunocytochemistry with an antiserum specific for Na+,K+-ATPase. The sectional areas of CCs increased markedly following SW transfer, whereas they remained small in the embryos and larvae maintained in FW. Scanning electron microscopy showed that the apical opening of CCs was enlarged in the fish transferred to SW. Transmission electron microscopy revealed enhanced cellular activity in SW, as evidenced by well-developed mitochondria and tubular systems. The CCs in SW frequently formed a multicellular complex, consisting of a main CC and one or two accessory cells. Accessory cells interdigitated with the main cells and extended their cytoplasmic processes to the apex of the main cell. The three-dimensional arrangement of the cells participating in the complex was identified by confocal laser scanning microscopy. Such complexes were rarely observed in FW fish. The activated CCs in the yolk-sac membrane in the SW fish probably function as ion-extruding sites during embryonic and larval stages until gill CCs become functional.
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  • 10
    ISSN: 1432-0878
    Keywords: Key words Brood pouch ; Epithelium ; Mitochondria-rich cell ; Na+ ; K+-ATPase ; Ion transport ; Pipefish ; Syngnathus schlegeli (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The brood pouch of the male pipefish (Syngnathus schlegeli) is a ventral organ located on the tail, with the anterior region closely associated with the genital pore. The embryos in the pouch are attached to highly vascularized placenta-like tissue which seals the pouch folds from inside during incubation. The epithelium of the placenta-like tissue consists of mitochondria-rich cells (MRCs) and pavement cells. Differences in MRC morphology in the brood pouch epithelium, the gills and the larval epidermis of the pipefish were examined by light and electron microscopy. Transmission electron microscopy revealed that the MRCs in the brood pouch and the gills shared common characteristics: the presence of numerous mitochondria packed among a well-developed tubular system and the close association of the basal parts with the capillaries running underneath the epithelia. The size of the apical opening of the elongate, flask-shaped brood pouch MRC was about one-tenth that of the apical pit of the gill MRC. The gill and larval epidermal MRCs formed a multicellular complex, in contrast to solitary brood pouch MRCs. The brood pouch MRCs were intensively stained by immunocytochemistry with an antiserum specific for Na+,K+-ATPase. The Na+ concentrations in the brood pouch were maintained near those in the serum rather than seawater during incubation. We conclude that the brood pouch MRCs function as an ion-transporting cell, absorbing ions from the brood pouch lumen, perhaps to protect the embryos from the hyperosmotic environment.
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