Publication Date:
2014-09-25
Description:
EstN2 is a novel α/β-hydrolase originating from the ammonia-oxidizing thaumarchaeonCandidatusNitrososphaera gargensis. The genome of the organism was sequenced and genes conferring putative lipolytic activity were amplified and cloned intoEscherichia colias a heterologous host. Through function-based screening, esterase and lipase activity was detected. A recombinant enzyme designated EstN2 was successfully expressed, purified and crystallized. The crystals belonged to space groupI2, with one molecule per asymmetric unit, and diffracted X-rays to 1.5 Å resolution.
Electronic ISSN:
2053-230X
Topics:
Biology
,
Chemistry and Pharmacology
,
Geosciences
,
Physics
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