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  • 1
    Electronic Resource
    Electronic Resource
    Localization of a chemotactic domain in human thrombin (1984)
    s.l. : American Chemical Society
    Biochemistry 23 (1984), S. 397-400 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00298a001
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  • 2
    Electronic Resource
    Electronic Resource
    Isomorphous heteropoly complexes containing various pairs of paramagnetic atoms. Exchange-coupled differing spins with absence of long-range magnetic interactions. New class of paramagnetic behavior. Theoretical treatment. Novel geometrical isomerism (1972)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 94 (1972), S. 5499-5501 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00770a057
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  • 3
    Electronic Resource
    Electronic Resource
    Endotoxin inhibition of macrophage-mediated bone resorption (1981)
    Springer
    Calcified tissue international 33 (1981), S. 269-275 
    Details
    ISSN: 1432-0827
    Keywords: Endotoxins ; Bone resorption ; Prostaglandins ; Lipopolysaccharides ; Macrophages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The mechanisms by which bacterial endotoxins (ETX) elicit bone loss in septic osteolytic lesions and in organ cultures of bone rudiments have never been clearly established. The possible mechanisms for ETX action include: (a) the stimulation of osteoclast proliferation; (b) the stimulation of synthesis of secondary agents known to elicit bone resorption, e.g., prostaglandins; (c) the stimulation of the resorptive activity of osteoclasts. In the absence of extant methods for isolating and culturing osteoclasts, we have explored the last possibility by evaluating the action of ETX in a bone resorption system consisting of a putative osteoclast precursor, the macrophage, cocultured with isotopically labeled devitalized bone. We have observed the following: 1. ETX from several species of bacteria (Escherichia coli, Shigella flexneri, andS. minnesota) suppress bone resorption (i.e.,45Ca release) mediated by thioglycollate-elicited peritoneal macrophages. This inhibition occurs at ETX concentrations as low as 0.5 µg/ml and is evident within the initial 24 h of incubation. In marked contrast, ETX does not alter the resorptive activity of resident peritoneal macrophages. 2. The suppression of bone resorption by ETX does not depend on the presence of serum complement nor is it a manifestation of reduced cell viability or cell bone-particle binding. Moreover, prolonged pretreatment of elicited cells with ETX does not reduce their subsequent resorptive activity. 3. The suppressive action of ETX is partially reversed by polymyxin B, an observation which implicates the lipid A component of ETX in the inhibitory process. 4. PGE1, PGE2, and indomethacin at concentrations as high as 10−5M do not alter macrophage-mediated resorption; neither does indomethacin modify the action of ETX when the two agents are used concurrently. However, PGE1 and PGE2 can mitigate the suppressive action of ETX. The latter result indicates but does not define a role for prostaglandin in the ETX phenomenon. We suggest the ETX elicits bone loss in vivo by stimulating osteoclast proliferation or prostaglandin synthesis, and not by directly evoking enhanced bone resorption by osteoclasts or other osteolytic cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409448
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  • 4
    Electronic Resource
    Electronic Resource
    The Occurrence, Growth-Temperature Relations and Nuclear Cytology of Protostelium mycophaga Olive and Stoianovitch (1967)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 14 (1967), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Protostelium mycophaga Olive and Stoianovitch was isolated from 7 of 19 plant sources collected in a 15-mile radius of Syracuse, New York. Two of these isolations were made from up-right, nondecomposed plants. Spore diameter measurements of 4 of the isolates gave values that agree with those published for this species. The optimum temperature for the growth of P. mycophaga on enriched corn meal agar and Rhodotorula mucilaginosa was 20 C. Limited growth occurred at 10 C and no growth was observed above 30 C.Mitosis is described from observations on living and stained preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1967.tb01449.x
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  • 5
    Electronic Resource
    Electronic Resource
    Resistivity of linear scratches in doped (100) n-type single-crystal silicon (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 63 (1988), S. 4568-4571 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Four-point probe measurements have been made on (100) n-type Czochralski silicon wafers of initial resistivities 0.016, 0.96, and 3.35 Ω cm. The probe tips straddled linear single scratches formed by a Vickers pyramid diamond. The diamond was dead-loaded with 0.25 N, and the scratches were made in a laboratory air environment with a relative humidity of 50%, as the silicon wafer was held at various elevated temperatures. The measurements show that the relative change in resistivity increases with temperature up to an optimum temperature, after which the resistivity decreases. The temperature at which the maximum occurs and at which the relative change in resistivity occurs depends on the initial resistivity of the wafers; the temperature at which the maximum change in relative resistivity occurs is 200 °C for the 0.016- and 0.96-Ω cm wafers and 250 °C for the 3.35-Ω cm wafer. The relative change between the undamaged wafer resistivity and the resistivity including the scratches for these same samples was 4%, 7%, and 9%. Scanning electron micrographs of the scratches showed that the scratch morphology also depended on the temperature; as the temperature is increased, the grooves become shallow and display evidence of ploughing. An analysis of the four-point probe geometry shows that the voltage in the four-point probe measurement depends on the size and the conductivity of the damage surrounding the scratches.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.340156
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  • 6
    Electronic Resource
    Electronic Resource
    Valence-band effective masses of GaAs (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 64 (1988), S. 447-450 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The density-of-states effective masses for the heavy-hole, light-hole, and split-off valence bands of GaAs have been calculated as a function of energy for each band. The calculations are based on a full k⋅p theory with the most recent values used for the matrix elements. Provision has been made for the effect of the split-off energy on the matrix elements of the split-off band. The results show important nonparabolicities which should be taken into account in modeling the valence band, and rational polynomial fits have been made for ease of computation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.341217
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  • 7
    Electronic Resource
    Electronic Resource
    Hormone-Like Activity of Human Thrombin (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 485 (1986), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1986.tb34595.x
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  • 8
    Electronic Resource
    Electronic Resource
    Boundary integral equation methods for two-dimensional models of crack-field interactions (1988)
    Springer
    Journal of nondestructive evaluation 7 (1988), S. 3-14 
    Details
    ISSN: 1573-4862
    Keywords: Electromagnetic nondestructive evaluation (NDE) ; crack detection ; eddy currents ; boundary integral equations ; boundary element method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract An introduction to the application of surface integral equation methods to the calculation of eddy current-flaw interactions is presented. Two two-dimensional problems are presented which are solved by the boundary integral equation method. Application of collocation methods reduces the problems to systems of linear algebraic equations. The first problem is that of a closed surface crack in a flat slab with an AC magnetic field parallel to the plane of the crack. The second is that of av-groove crack in the AC field of a pair of parallel wires placed parallel to the vertex of the crack. In both cases, maps of the current densities at the surface are displayed, as well as the impedance changes due to the cracks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00565772
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  • 9
    Electronic Resource
    Electronic Resource
    Inductive specificity of mineralized bone matrix in ectopic osteoclast differentiation (1982)
    Springer
    Calcified tissue international 34 (1982), S. 474-479 
    Details
    ISSN: 1432-0827
    Keywords: Osteoclast differentiation ; Giant cell formation ; Mineralized implants ; Bone implants ; Chorioallantoic membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The present report describes the first in a series of studies designed to identify the factor or factors responsible for eliciting osteoclast differentiation. Particles of mineralized and demineralized bone, hydroxyapatite (HA), and eggshell were grafted onto the chorioallantoic membranes (CAMs) of chick embryos. After 3 or 6 days, portions of CAMs with associated grafts were harvested, processed for light and electron microscopy, and examined for the presence of multinucleated giant cells with the morphological characteristics of osteoclasts. Light microscopic examination revealed that, within only 3 days, many particles of mineralized materials had become surrounded or engulfed by multinucleated giant cells. Ultrastructurally, all such cells possessed a vacuolated and mitochondriaenriched cytoplasm, but they differed in the nature of the contacts formed at the cell-particle interface. With eggshell, the cells developed filopodia but lacked clear zones and ruffled membranes. With HA, clear zones were evident but cytoplasmic extensions and membrane ruffling were absent. Implants of mineralized bone, however, elicited the formation of giant cells with prominent clear zones and ruffling of the plasma membrane like that observed in bonafide osteoclasts. In contrast, grafts of demineralized bone did not evoke giant cell formation but rather recruited two cell types morphologically akin to either fibroblasts or macrophages. We conclude that the factor(s) responsible for osteoclast differentiation resides specifically within bone matrix and is intimately associated with the mineral phase. Further, in response to such a factor(s), osteoclast differentiation can occur ectopically, outside of the developing vertebrate body.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411288
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  • 10
    Electronic Resource
    Electronic Resource
    Cell lineage in fracture healing in chimeric bone grafts (1979)
    Springer
    Calcified tissue international 27 (1979), S. 247-253 
    Details
    ISSN: 1432-0827
    Keywords: Osteoblast ; Osteoclast ; Osteoprogenitor cells ; Fracture ; Chimera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Previous studies have shown that differences in nuclear morphology are generally sufficient to determine the species origin of cells in interspecific grafts between the Japanese quail and domestic chicken. Most quail nuclei possess 1–3 large nucleolus-associated masses of heterochromatin. Chick cells, on the other hand, usually present a more diffuse, stippled distribution of nuclear heterochromatin. Quail embryonic limb rudiments, some with and some without established marrow cavities, were explanted and grown on the chorioallantoic membrane of the chick. Three to five days post-grafting, the explants were surgically fractured and allowed to heal. Tissues were collected and histologically processed during the latter period. The fractures healed completely within 5–6 days and no callus was established in the process. The nuclear staining pattern of the osteoblasts and osteocytes throughout the rudiments and at the fracture site indicated that they were derived from the graft. Possible sources for these cells included the periosteum, endosteum, and posthypertrophy chondrocytes. By contrast, most of the nuclei in the osteoclasts were chick-like and were apparently derived from cells originating in the host. Because the quail-like heterochromatin marker was normally present in a small number (2.5%) of chick osteoclast nuclei and was lacking in about 5% of native quail osteoclast nuclei, the precise extent of the participation of donor, i.e., quail bone and marrow stromal cells in osteoclast formation, could not be determined. However, the data suggest that in large measure the precursor cells for most osteoclasts were hematogenously derived and were carried to the grafted rudiments by the blood vascular system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441193
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