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  • 1
    Electronic Resource
    Electronic Resource
    Relationships between rhizoplane and rhizosphere bacteria and verticillium wilt resistance in potato (1985)
    Springer
    Archives of microbiology 140 (1985), S. 347-351 
    Details
    ISSN: 1432-072X
    Keywords: Bacteria ; Antagonists ; Verticillium dahliae ; Potato cultivars ; Resistance ; Populations ; Quantitative ; Qualitative-Roots ; Soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Six cultivars and breeding lines of potato (Solanum tuberosum) differing in susceptibility to verticillium wilt caused by Verticillium dahliae were studied with respect to quantitative and qualitative differences in the bacterial flora of their soil and rhizosphere-rhizoplane. Although, no association was observed between the types of bacteria that inhabited the soil or roots of wilt resistant and susceptible cultivars, quantitative differences were evident. These differences provide the first direct evidence that potato genotypes can influence bacterial populations. Bacterial populations were 9–25-fold higher on roots than in the adjacent soil. As the plants aged, the total number of rootcolonizing bacteria increased between 15 and 245%. Pseudomonas spp. were the most abundant microbes in the soil and rhizosphere-rhizoplane. The bacteria antagonistic to V. dahliae in vitro were identified as members of the genera Bacillus, Pseudomonas, Flavobacterium, and Gluconobacter. A statistically significant trend was evident toward the association of antagonistic bacteria with the more resistant potato cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446976
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of a new Agrobacterium tumefaciens strain from alfalfa (Medicago sativa L.) (1998)
    Springer
    Archives of microbiology 169 (1998), S. 381-386 
    Details
    ISSN: 1432-072X
    Keywords: Key words Agrobacterium tumefaciens ; Alfalfa ; Medicago sativa ; Ti plasmid ; Virulence ; Octopine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Agrobacterium tumefaciens strain 1D1609 is reported here as the first field isolate from alfalfa (Medicago sativa L.). Unlike well-characterized A. tumefaciens strains such as C58 and Ach5, strain 1D1609 is highly virulent on alfalfa and has a distinctive host range. Interestingly, strain 1D1609 is naturally resistant to kanamycin and spectinomycin. The Ti plasmid in strain 1D1609 is an octopine-type; thus, tumors formed by strain 1D1609 synthesize octopine, which is utilized by the bacterium as a sole carbon source. Reciprocal exchange of Ti plasmids between strains 1D1609 and C58 showed that both chromosomal and Ti plasmid genes in strain 1D1609 contribute specifically to tumor formation on alfalfa. In addition, the nondormant CUF101 alfalfa cultivar from which strain 1D1609 was isolated was significantly more susceptible to all Agrobacterium strains tested than was the dormant Agate cultivar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050586
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  • 3
    Electronic Resource
    Electronic Resource
    Increase of Plant Virus Infection by Magnesium in the Presence of Phosphate (1963)
    [s.l.] : Nature Publishing Group
    Nature 197 (1963), S. 925-926 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The enhancing action of magnesium on infection has been observed with poliovirus5 and with the yield of rll mutants of bacteriophage T4 (ref. 6). Magnesium in combination with glycine has been reported to stabilize potato yellow-dwarf virus7, but not barley stripe mosaic virus8. Magnesium has also ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/197925a0
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  • 4
    Electronic Resource
    Electronic Resource
    The Tumor-Inducing Substance of Agrobacterium Tumefaciens (1976)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Phytopathology 14 (1976), S. 265-308 
    Details
    ISSN: 0066-4286
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.py.14.090176.001405
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  • 5
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of the virG locus of the Agrobacterium tumefaciens plasmid pTiC58 (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 1 (1987), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The nucleotide sequence of the virG locus of the nopaline type plasmid pTiC58 of Agrobacterium tumefaciens has been determined. It contains an open reading frame (ORF) of 759 nucleotides and has 77% homology to the virG sequences of octopine type plasmids. Differences between the sequences of the two types of Ti plasmids in the region of virG are located predominantly outside the ORF. The amino acid sequences inferred from the two virG genes show 80% homology to each other and each shows the same moderate homologies to amino acid sequences derived from genes in a family of two-component regulatory systems. Specific differences in nucleotide and amino acid sequences as well as a structure-function model for the gene product are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1987.tb01937.x
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  • 6
    Electronic Resource
    Electronic Resource
    Biosurfactants produced by Pseudomonas fluorescens and soft-rotting of harvested florets of broccoli and cauliflower (2004)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 53 (2004), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Mini-Tn5 transposon mutagenesis of a soft-rotting isolate of Pseudomonas fluorescens strain 123 produced six mutant phenotypes with altered pathogenicity and inability to produce a biosurfactant involved in dispersing P. fluorescens cells in a surface aqueous environment. The mutants isolated showed in vivo growth characteristics identical to those of the wild type, but variations in their ability to reduce surface tension of water and to grow in liquid medium containing hexadecane. One of these mutants (EG3) with reduced pathogenicity on broccoli and cauliflower exhibited a restored pathogenic phenotype when complemented with a 7·1-kb segment of P. fluorescens genomic DNA. The loss of aqueous-surface-tension activity in mutants suggests that a biosurfactant may contribute to pathogenicity and enhance pathogen invasion of host tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0032-0862.2004.01048.x
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  • 7
    Electronic Resource
    Electronic Resource
    Regulation of the virC and virD promoters of pTiC58 by the ros chromosomal mutation of Agrobacterium tumefaciens (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The virC and virD operons of the virulence region of the Ti plasmid are highly regulated, requiring a transcriptional regulator that is encoded by virG and is activated by the product of virA and plant phenolics such as acetosyringone. Full expression of virC and virD of octopine and nopaline Ti plasmids is also obtained by a mutation in the ros gene of the Agrobacterium tumefaciens chromosome. S1-nuclease analysis, in vitro transcription, and DNase I protection experiments with A. tumefaciens RNA polymerase revealed virD promoters tandemly arranged, both of which are functional in the Ros mutant, while only one is functional in the presence of acetosyringone. A third (overlapping) promoter appears to be responsible for transcription of virC. Expression of virC and virD appears to be modulated by factors within the bacterium by means of a mechanism that is independent of factors produced during infection of the host plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1988.tb00043.x
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  • 8
    Electronic Resource
    Electronic Resource
    virG of Agrobacterium tumefaciens plasmid pTiC58 encodes a DNA-binding protein (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 3 (1989), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Virulence genes of the Agrobacterium tumefaciens Ti plasmid are positively regulated by the products of virA and virG. To study the DNA-binding properties of the VirG protein, a translational fusion between virG and the frpEgene of Escherichia coli was constructed, and antiserum was raised against the encoded fusion protein. Using this antiserum, a protein of Mr= 29000, a size similar to that calculated from the virG nucleotide sequence, was detected in an E. coli strain harbouring a virG expression vector. Both the VirG protein and the fusion protein were found, by filter-binding and gel retardation analyses, to bind DNA nonspecifically. These data support an existing model for the two-component regulatory systems of bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1989.tb00186.x
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  • 9
    Electronic Resource
    Electronic Resource
    A gene near the plasmid pSa origin of replication encodes a nuclease (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We have cloned and sequenced a gene (nuc) from the IncW plasmid pSa which shows amino acid sequence similarity to staphylococcal nuclease (EC 3.1.4.7) and to the parB locus of plasmid RP4. The 525bp open reading frame encodes a 174-amino-acid potential polypeptide of 19.7kDa. Expression of the gene was confirmed using an in vitro transcription-translation assay which produced a protein of identical size. Nuclease activity was demonstrated using DNA as the substrate in toluidine blue-DNA agar plates. The deduced amino acid sequence revealed a signal sequence, and TnphoA insertion within the open reading frame indicated that a portion of the protein is transported across the bacterial cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb01497.x
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of the virA virulence gene of the nopaline plasmid, pTiC58, of Agrobacterium tumefaciens (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 4 (1990), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1990.tb02026.x
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