Publication Date:
1994-06-10
Description:
The alpha 1-adrenergic receptors activate a phospholipase C enzyme by coupling to members of the large molecular size (approximately 74 to 80 kilodaltons) G alpha h family of guanosine triphosphate (GTP)-binding proteins. Rat liver G alpha h is now shown to be a tissue transglutaminase type II (TGase II). The transglutaminase activity of rat liver TGase II expressed in COS-1 cells was inhibited by the nonhydrolyzable GTP analog guanosine 5'-O-(3-thiotriphosphate) or by alpha 1-adrenergic receptor activation. Rat liver TGase II also mediated alpha 1-adrenergic receptor stimulation of phospholipase C activity. Thus, G alpha h represents a new class of GTP-binding proteins that participate in receptor signaling and may be a component of a complex regulatory network in which receptor-stimulated GTP binding switches the function of G alpha h from transglutamination to receptor signaling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nakaoka, H -- Perez, D M -- Baek, K J -- Das, T -- Husain, A -- Misono, K -- Im, M J -- Graham, R M -- New York, N.Y. -- Science. 1994 Jun 10;264(5165):1593-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cardiovascular Biology, Cleveland Clinic Foundation, OH 44195.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7911253" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Cell Line
;
Epinephrine/pharmacology
;
GTP-Binding Proteins/chemistry/genetics/*metabolism
;
Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
;
Guinea Pigs
;
Inositol Phosphates/metabolism
;
Liver/enzymology
;
Molecular Sequence Data
;
Prazosin/pharmacology
;
Rats
;
Receptors, Adrenergic, alpha/genetics/*metabolism
;
*Signal Transduction
;
Transfection
;
Transglutaminases/chemistry/genetics/*metabolism
;
Type C Phospholipases/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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