ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Diode array detectors (DAD)
;
Toxicology
;
Automated solute identification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The possibilities and limitation of automated solute identification via UV/VIS spectroscopy in HPLC are demonstrated and applications in toxicologic analysis are described. A standard isocratic system is used. The influence of various chromatographic parameters on retention time is shown. A retention time window of ±15% is required. In addition to retention time, derived spectral data are calculated (minima and maxima of derivatives, area counts under standardized spectral ranges, absorption at various wavelength) and used for positive solute identification. It is possible to identify solutes with a high degree of certainty in urine and plasma if their concentration is at least 10 ppm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02261012
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