ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Mouse anti-chicken monoclonal antibodies were raised against an avian myeloblastosis virus (AMV)-transformed myeloblastic leukemic cell line. One monoclonal antibody, S1-37 (lgG2a), reacted with producer and nonproducer myeloblastic leukemia cell lines transformed by AMV and by E-26 virus, but it did not react with chicken fibroblasts infected with RAV-2, MAV-2, MAV-1, or RAV-7, S1-37 also did not react with normal chicken hemopoietic cells, except for yolk sack macrophages and a small population of embryonal and adult bone marrow cells that morphologically resembled macrophages.Cytotoxicity studies of GM-CFU, the normal stem cell population of the granulocytic macrophage lineage, indicated that these cells lack the surface antigen recognized by S1-37. Immunoprecipitation studies of 125I surfacelabeled myeloblastic leukemic cells indicated that S1-37 binds a 42,000 Mr polypeptide. The possible role of this polypeptide in the process of transformation and differentiation of chicken myeloid cells is discussed.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1041130513
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