ISSN:
1432-0983
Keywords:
Basidiomycete
;
Fungicide resistance
;
Succinate dehydrogenase
;
Iron-sulphur protein
;
Complex II
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A gene which confers resistance to the systemic fungicide carboxin (Cbx) has been isolated from the maize pathogen, Ustilago maydis, by transferring a plasmid gene library from a Cbx-resistant mutant strain into a sensitive strain and selecting for expression of the resistance gene. Five plasmids, rescued from transformants which exhibited enhanced resistance to Cbx, were shown to have DNA inserts with common restriction enzyme fragments. All the plasmids transformed a sensitive U. maydis strain to Cbx resistance. The gene (Cbx r ), sub-cloned on a 3.2 kb EcoR1-HindIII fragment, transformed U. maydis to Cbx resistance at frequencies similar to those obtained with the bacterial Hygromycin B resistance (HygB r ) gene. The sequence of the Cbx r gene showed a high degree of homology to succinate dehydrogenase (EC 1.3.99.1) iron-sulphur subunit genes from other organisms.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00312739
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