Publication Date:
2016-12-02
Description:
Introduction: Preclinical researches on the use of mesenchymal stem cells (MSCs) transplantation to treat hypoxic-ischemic (HI) brain damage have received some encouraging results. However, the insufficient migration of active cells to damaged tissues has limited their potential therapeutic effects. There is a good evidence that hypoxia inducible factor-1 alpha (HIF-1α) promotes the viability and migration of cells. This study was designed to investigate whether overexpression of HIF-1α in MSCs could be effective to enhance the therapeutic efficiency. Methods: MSCs were obtained from rat femoral and tibial bone marrow, and cells with HIF-1α overexpression were gained by recombinant lentiviral vector. The proliferation and migration of MSCs were assessed by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and transwell culture system. Additionally, the therapeutic efficiency of MSCs after transplantion on HI brain damage in rats were investigated by Morris water maze test and histological examination to analyse the cognitive and pathological changes, and the recruitment in the hippocampus was also observed microscopically. Results: (1) As evidenced by phase contrast and fluorescence microscopy, more than 90% of the cells were GFP-positive when infected with the HIF-1α or GFP lentiviral, and the protein expression of HIF-1α in HIF-1α transduced MSCs was approximately 2-fold that in normal MSCs and GFP transduced MSCs (P
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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