ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Nonwoven nanofiber matrices were prepared by electrospinning a solution of silk fibroin(SF) dissolved either in formic acid or in 1,1,1,3,3,3-hexafluoro-2-isopropyl alcohol (HFIP). Themean diameter of the electrospun nanofibers prepared from SF dissolved in formic acid was 80 nmwith a unimodal size distribution, which was smaller than those prepared from HFIP (380 nm). SFnanofibers were then treated with an aqueous methanol solution, and structural changes due tosolvent-induced crystallization of SF were investigated using IR and 13C solid-state CP/MAS NMRspectroscopy. SF nanofibers prepared from formic acid were found to have a higher proportion ofβ-sheet conformations than those prepared from HFIP. Methanol treatment provided a fast andeffective means to alter the secondary structure of both types of SF nanofibers from a random coilform to a β-sheet form. As demonstrated in the present study, this approach to controlling thedimensions and secondary structure of proteins using various solvents may be useful for the designand tailoring of materials for biomedical applications, especially for tissue engineering applications
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.813.pdf
Permalink