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  • 1
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    Genomic Predictions and Genome-Wide Association Study of Resistance Against Piscirickettsia salmonis in Coho Salmon (Oncorhynchus kisutch) Using ddRAD Sequencing (2018)
    Genetics Society of America (GSA)
    Details
    Publication Date: 2018-03-29
    Description: Piscirickettsia salmonis is one of the main infectious diseases affecting coho salmon ( Oncorhynchus kisutch ) farming, and current treatments have been ineffective for the control of this disease. Genetic improvement for P. salmonis resistance has been proposed as a feasible alternative for the control of this infectious disease in farmed fish. Genotyping by sequencing (GBS) strategies allow genotyping of hundreds of individuals with thousands of single nucleotide polymorphisms (SNPs), which can be used to perform genome wide association studies (GWAS) and predict genetic values using genome-wide information. We used double-digest restriction-site associated DNA (ddRAD) sequencing to dissect the genetic architecture of resistance against P. salmonis in a farmed coho salmon population and to identify molecular markers associated with the trait. We also evaluated genomic selection (GS) models in order to determine the potential to accelerate the genetic improvement of this trait by means of using genome-wide molecular information. A total of 764 individuals from 33 full-sib families (17 highly resistant and 16 highly susceptible) were experimentally challenged against P. salmonis and their genotypes were assayed using ddRAD sequencing. A total of 9,389 SNPs markers were identified in the population. These markers were used to test genomic selection models and compare different GWAS methodologies for resistance measured as day of death (DD) and binary survival (BIN). Genomic selection models showed higher accuracies than the traditional pedigree-based best linear unbiased prediction (PBLUP) method, for both DD and BIN. The models showed an improvement of up to 95% and 155% respectively over PBLUP. One SNP related with B-cell development was identified as a potential functional candidate associated with resistance to P. salmonis defined as DD.
    Electronic ISSN: 2160-1836
    Topics: Biology
    Published by Genetics Society of America (GSA)
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  • 2
    Electronic Resource
    Electronic Resource
    Role of sulfatide on phosphoenzyme formation and ouabain binding of the (Na^+ + K^+) ATPase
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 238 (1985), S. 558-564 
    Details
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-9861(85)90200-0
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of metal ions on the activity of cascein kinase II from Xenopus laevis
    Amsterdam : Elsevier
    FEBS Letters 315 (1993), S. 173-177 
    Details
    ISSN: 0014-5793
    Keywords: CaScin kinase II ; CaScin kinase II α and β subunits ; Divalent metal ion ; Xenopus laevis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(93)81157-U
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  • 4
    Electronic Resource
    Electronic Resource
    Activity of the E^7^5E^7^6 mutant of the α subunit of casein kinase II from Xenopus laevis
    Amsterdam : Elsevier
    FEBS Letters 339 (1994), S. 93-96 
    Details
    ISSN: 0014-5793
    Keywords: Casein kinase II ; Mutated α subunit ; Poly U ; Quercetin ; Xenopus laevis, Heparin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(94)80392-7
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  • 5
    Electronic Resource
    Electronic Resource
    The cDNAs coding for the α- and β-subunits of Xenopus laevis casein kinase II
    Amsterdam : Elsevier
    FEBS Letters 297 (1992), S. 280-284 
    Details
    ISSN: 0014-5793
    Keywords: Amphibian oocyte ; Protein kinase ; Protein phosphorylation ; Sequence motif ; Xenopus laevis, casein kinase II
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(92)80556-V
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  • 6
    Electronic Resource
    Electronic Resource
    Relationship between the length of sperm preincubation and zona penetration in the golden hamster: A scanning electron microscopy study (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 9 (1984), S. 31-43 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoon ; zona pellucida ; scanning electron microscope ; hamster ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Hamster spermatozoa are able to fertilize a high percentage of zona-intact hamster oocytes when they are preincubated for 2 hr in a chemically defined medium. From this time on, the longer the preincubation time the lower the percentage penetration. Spermatozoa preincubated for 6 or more hr are unable to cross the zona pellucida, retaining however their ability to fuse with zona-free hamster oocytes.Zona-intact hamster oocytes, as described above, were observed with the scanning electron microscope. When the oocytes were inseminated with spermatozoa preincubated for 1 to 5 hr the outer surface of the zona showed the penetrating spermatozoa and the sperm tracks made by those that failed to cross it. With longer preincubation times no penetrating spermatozoa were observed, and very few sperm tracks were present on the outer surface of the zona.Control experiments showed that neither eggs, spermatozoa, nor fertilization were affected by the medium recovered after long preincubations.These results show that care should be taken regarding the preincubation time when using the in-vitro fertilization technique.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120090104
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  • 7
    Electronic Resource
    Electronic Resource
    Scanning electron microscope study of in vitro prepenetration gamete interactions (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 11 (1985), S. 121-131 
    Details
    ISSN: 0148-7280
    Keywords: zona pellucida ; scanning electron microscope ; gamete interactions ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Hamster and mouse capacitated spermatozoa were interacted in vitro with hamster and mouse eggs in homologous and heterologous combinations. Also, fertilized and trypsin treated unfertilized hamster eggs, and unfertilized rat eggs were made to interact with capacitated hamster spermatozoa. The surface of the zona pellucida was then examined with the scanning electron microscope. It was found that sperm attachment, followed by sperm binding and penetration through the zona pellucida, was observed only when homologous gamete combinations were used. Binding of the spermatozoa to the zona was evidenced by the lytic effect of the acrosomal enzymes on the zona substance. When fertilized eggs and trypsin-treated unfertilized hamster eggs were mixed with capacitated hamster spermatozoa as well as in the heterologous gamete combinations, we found that the spermatozoa were able to establish attachment but not binding. Under these conditions the outer surface of the zona pellucida was never found to have penetration tracks made by the spermatozoa. Failure of heterologous spermatozoa to cross the foreign zona pellucida is believed to be associated with the inability of the foreign spermatozoa to establish binding and to the inability of their acrosomal enzymes to digest the zona. A similar mechanism is believed to work in zona-reacted and in trypsin-treated hamster eggs inseminated in vitro with homologous spermatozoa.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120110203
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of in vivo oocyte aging on sperm chromatin decondensation in the golden hamster (1986)
    New York, NY : Wiley-Blackwell
    Gamete Research 14 (1986), S. 347-354 
    Details
    ISSN: 0148-7280
    Keywords: aged eggs ; parthenogenesis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Aged spontaneously activated hamster oocytes recovered from adult females 18 and 24 hours after ovulation were at the pronuclear stage. These oocytes and fresh controls were inseminated in vitro with capacitated hamster spermatozoa and observed with the phase-contrast microscope. The percentage of fertilization in fresh control oocytes was 98%, as compared to 36% and 18% when the oocytes were recovered 18 and 24 hours after ovulation, respectively. The mean number of sperm decondensations per egg in control oocytes was 10, and in the aged ones it was 0.69 and 0.12 when the oocytes were recovered 18 and 24 hours after ovulation, respectively. When similarly treated oocytes were studied with scanning and transmission electron microscopy, it was found that the degree of gamete membrane fusion was greater than that observed with the phase-contrast microscope, but that most of the spermatozoa failed to decondense the chromatin. We suggest that parthenogenetic oocytes at the pronuclear stage are in a similar stage of the cell cycle as in fertilized eggs, in which the cytoplasm does not have the ability to decondense the sperm chromatin.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120140407
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  • 9
    Electronic Resource
    Electronic Resource
    Scanning electron microscope study of human cervical mucus (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 12 (1985), S. 85-89 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoon ; cervical mucus ; human ; scanning electron microscope ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The ultrastructure of human cervical mucus was studied by scanning electron microscopy. In fertile women, naturally occurring midcycle cervical mucus showed an arrangement of parallel fibers oriented along the main axis of the mucus sample. Sperm migration through a column of cervical mucus in vitro was also studied. It was found that sperm present among the longitudinal fibers were oriented parallel to the them and to the main axis of the mucus sample.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120120110
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