Publication Date:
2009-11-20
Description:
Abstract 2835 Poster Board II-811 Introduction: We have previously shown that Regulatory T-cells (TReg cells) are increased in the peripheral blood (PB) and bone marrow (BM) of patients with multiple myeloma (MM), related to the stage of disease. Therefore, to investigate whether the tumour cells in MM generate and/or expand TReg cells as a method of immuno-surveillance avoidance, we developed an in vitro model of TReg cell generation. Materials and Methods: PBMCs from PB of healthy donors were co-cultured with human myeloma cell line, U266B, for 7 days. Using a sequential gating strategy, TReg cells identified as CD4+/CD25+/FoxP3+ T-cells were expressed as a percentage of the CD4+ T-cell population. Experiments were repeated after CD25 depletion ± CD4 selection and using transwells to examine cell contact dependence. The tumour generated TReg cells (tTReg cells) were isolated on day 7 by FACS and compared with naturally occurring TReg cells (nTReg cells). Results: Co-culture of unselected PBMC's demonstrated a non-significant increase in TReg cells compared with controls (n=8; controls 3.8±0.8%, co-culture 6.7±1%, p=0.06). However, co-culture of CD4+CD25− T-cells with U266 resulted in a significant increase in tTReg cells (n=15; control 0.6±0.3%; co-culture 31.5±3.6%, p
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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